Mouse monoclonal antibody

Anti-Complement component C4c

CAT NO: CAM 072-18 | Application: ELISA, WB, IP, IHC, IF

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Product Summary

Cat. No.
CAM 072-18
Specificity
CAM 072-18 binds human complement factor C4c.
Immunogen
Synthetic peptide consisting of the last 15C-terminal residues of the C4c a4-domain (Cys-EELVYELNPLDHRGR).`
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG2b/k
Clone Number
99-72-18
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. CAM 072-18-04 1 mL: Cat. No. CAM 072-18-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
C4c is a compoment in the Complement cascade and is a degradation product of C4b which is cleaved by C4b/C3b inactivator to yield C4d and C4c (soluble). C4c consists of a g-chain (30 kDa), a b-chain (75 kDa), and two a-domains denoted a3 (27 kDa) and a4 (14 kDa). C4c reacts with C1 and C2 to form C3 convertase of the classic activation pathway.

Application

ELISA: CAM 072-18 was used in ELISA. (1) When CAM 072-18 (4 μg/mL) was used as a capture antibody, biotinylated HYB 162-02 (0.4 μg/mL) was used as the detection antibody in order to measure C4c.

WB: CAM 072-18 was used in Western blot. (1)

IP: CAM 072-18 was used in immunpercipitation. (1)

IHC: CAM 072-18 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:600.

IHC staining of human kidney with CAM 072-18. Strong staining of veins of nephritis kidney. Dilution: 1:600

IHC staining of human liver with CAM 072-18. Cytoplasm of hepatocytes moderately labelled and plasma of veins strongly in liver tissue. Dilution: 1:600

IHC staining in human tonsil with CAM 072-18. Plasma in lumen of veins is stained strongly as well as follicular dendritic cells in germinal center of tonsil. Dilution: 1:600

IF: CAM 072-18 was used in IF with a dilution of 1:200.

IF staining of human kidney with CAM 072-18. Strong staining of veins of nephritis kidney. Dilution: 1:200

IF staining in human tonsil with CAM 072-18. Plasma in lumen of veins is stained strongly in tonsil tissue. Dilution: 1:200

Scientific References

1. Pilely K, Skjodt MO, Nielsen C, Andersen TE, Aabom AL, Vitved L, Koch C, Skjodt K, Palarasah Y (2014) A specific assay for quantification of human C4c by use of an anti-C4c monoclonal antibody. J Immunol Methods 405:87-96.

Cat. No.
CAM 072-18
Specificity
CAM 072-18 binds human complement factor C4c.
Immunogen
Synthetic peptide consisting of the last 15C-terminal residues of the C4c a4-domain (Cys-EELVYELNPLDHRGR).`
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG2b/k
Clone Number
99-72-18
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. CAM 072-18-04 1 mL: Cat. No. CAM 072-18-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
C4c is a compoment in the Complement cascade and is a degradation product of C4b which is cleaved by C4b/C3b inactivator to yield C4d and C4c (soluble). C4c consists of a g-chain (30 kDa), a b-chain (75 kDa), and two a-domains denoted a3 (27 kDa) and a4 (14 kDa). C4c reacts with C1 and C2 to form C3 convertase of the classic activation pathway.

ELISA: CAM 072-18 was used in ELISA. (1) When CAM 072-18 (4 μg/mL) was used as a capture antibody, biotinylated HYB 162-02 (0.4 μg/mL) was used as the detection antibody in order to measure C4c.

WB: CAM 072-18 was used in Western blot. (1)

IP: CAM 072-18 was used in immunpercipitation. (1)

IHC: CAM 072-18 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:600.

IHC staining of human kidney with CAM 072-18. Strong staining of veins of nephritis kidney. Dilution: 1:600

IHC staining of human liver with CAM 072-18. Cytoplasm of hepatocytes moderately labelled and plasma of veins strongly in liver tissue. Dilution: 1:600

IHC staining in human tonsil with CAM 072-18. Plasma in lumen of veins is stained strongly as well as follicular dendritic cells in germinal center of tonsil. Dilution: 1:600

IF: CAM 072-18 was used in IF with a dilution of 1:200.

IF staining of human kidney with CAM 072-18. Strong staining of veins of nephritis kidney. Dilution: 1:200

IF staining in human tonsil with CAM 072-18. Plasma in lumen of veins is stained strongly in tonsil tissue. Dilution: 1:200

1. Pilely K, Skjodt MO, Nielsen C, Andersen TE, Aabom AL, Vitved L, Koch C, Skjodt K, Palarasah Y (2014) A specific assay for quantification of human C4c by use of an anti-C4c monoclonal antibody. J Immunol Methods 405:87-96.

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