Mouse monoclonal antibody

Anti-Complement component C5a/C5a(desArg) anaphylatoxin (human)

CAT NO: GAU 025-05 | Application: ELISA, WB

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Product Summary

Cat. No.
GAU 025-05
Specificity
GAU 025-05 reacts with human C5a/C5a(desArg).
Immunogen
Purified human C5a
Host
Mouse
Positive Species Reactivity
Human, Baboon
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
725
Clone Number
C17/5
Epitope Specificity
GAU 025-05 binds to a neoepitope exposed on C5a/C5a(desArg). (1)
Unit Size
400 µL: Cat. No. GAU 025-05-04 1 mL: Cat. No. GAU 025-05-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Complement C5a is generated in the circulation by the activation of complement. It is a so-called anaphylatoxin of 74 amino-acid residues generated by the action of C5 convertase, which cleaves C5 into C5a and C5b. It is rapidly inactivated by serum carboxypeptidase B, which removes the C-terminal arginine residue generating C5a(desArg). (1)

Application

ELISA: GAU 025-05 reacts well with C5a/C5a(desArg). A strong reaction is seen in ELISA with recombinant C5a coated onto the microtiter well. (1)

WB: GAU 025-05 can be used in Western blotting. (1)

Scientific References

1. Oppermann M, Schulze M, Götze O (1991) A sensitive enzyme immunoassay for the quantitation of human C5a/C5a(desArg) anaphylatoxin using a monoclonal antibody with specificity for a neoepitope. Complement Inflamm. 8:13-24.

Cat. No.
GAU 025-05
Specificity
GAU 025-05 reacts with human C5a/C5a(desArg).
Immunogen
Purified human C5a
Host
Mouse
Positive Species Reactivity
Human, Baboon
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
725
Clone Number
C17/5
Epitope Specificity
GAU 025-05 binds to a neoepitope exposed on C5a/C5a(desArg). (1)
Unit Size
400 µL: Cat. No. GAU 025-05-04 1 mL: Cat. No. GAU 025-05-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Complement C5a is generated in the circulation by the activation of complement. It is a so-called anaphylatoxin of 74 amino-acid residues generated by the action of C5 convertase, which cleaves C5 into C5a and C5b. It is rapidly inactivated by serum carboxypeptidase B, which removes the C-terminal arginine residue generating C5a(desArg). (1)

ELISA: GAU 025-05 reacts well with C5a/C5a(desArg). A strong reaction is seen in ELISA with recombinant C5a coated onto the microtiter well. (1)

WB: GAU 025-05 can be used in Western blotting. (1)

1. Oppermann M, Schulze M, Götze O (1991) A sensitive enzyme immunoassay for the quantitation of human C5a/C5a(desArg) anaphylatoxin using a monoclonal antibody with specificity for a neoepitope. Complement Inflamm. 8:13-24.

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Product Specification

Non-hazardous statement (SDS)

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