Mouse monoclonal antibody

Anti-Complement component C5a/C5a(desArg) anaphylatoxin (human)

CAT NO: GAU 025-05 | Application: ELISA, WB

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Product Summary

Cat. No.

GAU 025-05

Specificity

GAU 025-05 reacts with human C5a/C5a(desArg).

Immunogen

Purified human C5a

Host

Mouse

Positive Species Reactivity

Human, Baboon

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

725

Clone Number

C17/5

Epitope Specificity

GAU 025-05 binds to a neoepitope exposed on C5a/C5a(desArg). (1)

Unit Size

400 µL: Cat. No. GAU 025-05-04 1 mL: Cat. No. GAU 025-05-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Complement C5a is generated in the circulation by the activation of complement. It is a so-called anaphylatoxin of 74 amino-acid residues generated by the action of C5 convertase, which cleaves C5 into C5a and C5b. It is rapidly inactivated by serum carboxypeptidase B, which removes the C-terminal arginine residue generating C5a(desArg). (1)

Application

ELISA: GAU 025-05 reacts well with C5a/C5a(desArg). A strong reaction is seen in ELISA with recombinant C5a coated onto the microtiter well. (1)

WB: GAU 025-05 can be used in Western blotting. (1)

Scientific References

1. Oppermann M, Schulze M, Götze O (1991) A sensitive enzyme immunoassay for the quantitation of human C5a/C5a(desArg) anaphylatoxin using a monoclonal antibody with specificity for a neoepitope. Complement Inflamm. 8:13-24.