Mouse monoclonal antibody

Anti-Complement factor H (human, ß1H-globulin)

CAT NO: GAU 020-03 | Application: ELISA, WB

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Product Summary

Cat. No.

GAU 020-03

Specificity

GAU 020-03 is specific for complement factor H in human serum and plasma. (1)

Immunogen

Human Factor H (1)

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

3075

Clone Number

L20/3

Epitope Specificity

Epitope specificity differs from that of GAU 018-03. (1)

Unit Size

400 µL: Cat. No. GAU 020-03-04 1 mL: Cat. No. GAU 020-03-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Factor H is a regulatory factor of the alternative pathway of complement activation. Factor H is a glycoprotein consisting of a single polypeptide chain with a molecular mass of 150 kDa.

Application

ELISA: GAU 018-03 can be used as a capture antibody in sandwich ELISA with GAU 020-03 as a biotinylated detection antibody. (1, 2) This antibody pair specifically detects full length 150 kDa factor H and does not cross-react with the truncated forms of factor H. (1)

Immunoblot (human serum) with GAU 018-03 and GAU 020-03.

WB: GAU 020-03 can be used in Western blotting.

Human duodenum stained with GAU 018-03.

Scientific References

1. Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190.

2. Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int. 40:939-947.