Mouse monoclonal antibody

Anti-Complement factor H (human, ß1H-globulin)

CAT NO: GAU 018-03 | Application: ELISA, WB, IHC

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Product Summary

Cat. No.
GAU 018-03
Specificity
GAU 018-03 is specific for complement factor H in human serum and plasma. (1)
Immunogen
Human Factor H (1)
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
3075
Clone Number
C18/3
Epitope Specificity
Epitope specificity differs from that of GAU 020-03. (1)
Unit Size
400 µL: Cat. No. GAU 018-03-04 1 mL: Cat. No. GAU 018-03-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Factor H is a regulatory factor of the alternative pathway of complement activation. Factor H is a glycoprotein consisting of a single polypeptide chain with a molecular mass of 150 kDa.

Application

ELISA: GAU 018-03 can be used as a capture antibody in sandwich ELISA with biotinylated GAU 020-03 as a detection antibody. This antibody pair specifically detects full length 150 kDa factor H and does not cross-react with the truncated forms of factor H. (1, 2)

WB: GAU 018-03 can be used in Western blotting.

Immunoblot (human serum) with GAU 018-03 and GAU 020-03.

IHC: GAU 018-03 can be used in Immunohistochemistry.

Human duodenum stained with GAU 018-03.

Scientific References

1. Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190.

2. Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int. 40:939-947.

Cat. No.
GAU 018-03
Specificity
GAU 018-03 is specific for complement factor H in human serum and plasma. (1)
Immunogen
Human Factor H (1)
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
3075
Clone Number
C18/3
Epitope Specificity
Epitope specificity differs from that of GAU 020-03. (1)
Unit Size
400 µL: Cat. No. GAU 018-03-04 1 mL: Cat. No. GAU 018-03-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Factor H is a regulatory factor of the alternative pathway of complement activation. Factor H is a glycoprotein consisting of a single polypeptide chain with a molecular mass of 150 kDa.

ELISA: GAU 018-03 can be used as a capture antibody in sandwich ELISA with biotinylated GAU 020-03 as a detection antibody. This antibody pair specifically detects full length 150 kDa factor H and does not cross-react with the truncated forms of factor H. (1, 2)

WB: GAU 018-03 can be used in Western blotting.

Immunoblot (human serum) with GAU 018-03 and GAU 020-03.

IHC: GAU 018-03 can be used in Immunohistochemistry.

Human duodenum stained with GAU 018-03.

1. Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190.

2. Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int. 40:939-947.

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Product Specification

Non-hazardous statement (SDS)

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