Product Summary

Cat. No.
ABS 054-14B
Specificity
ABS 054-14 binds human Gastric Inhibitory Peptide (GIP) (3-42).
Immunogen
Synthetic human gastric inhibitory polypeptide
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Gene ID
2695
Clone Number
14
Epitope Specificity
The epitope in in the region of GIP (3-42).
Unit Size
150 µL: Cat. No. ABS 054-14B-015 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide
Conjugation
Biotin
Storage
2-8ºC without exposure to light. No precautions necessary during handling.
Target
Human gastric inhibitory polypeptide (GIP) is a 42 amino acid peptide belonging to the glucagon-secretin family of peptide hormones. It is secreted by endocrine cells in the duodenal mucosa and stimulates glucose-dependent insulin secretion as well as GLP-1 release from more distal endocrine (L) cells in the intestinal mucosa. GIP shows amino-acid sequence similarities to glucagon, GLP-1 and GLP-2 (from approximately 50% identity for glucagon to 30% identity for GLP-2).

Application

ELISA: ABS 057-25 (as capture antibody) forms a sandwich ELISA pair with ABS 054-14B (as biotinylated detection antibody) for measuring GIP. Data suggest that this matched antibody pair is the most sensitive pair for measuring GIP (3-42). The detection limit obtained with our non-optimized buffer system is up to 1 ng/mL of GIP (3-42). Cross-reaction with GIP (1-42) is around 2%.

Cat. No.
ABS 054-14B
Specificity
ABS 054-14 binds human Gastric Inhibitory Peptide (GIP) (3-42).
Immunogen
Synthetic human gastric inhibitory polypeptide
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Gene ID
2695
Clone Number
14
Epitope Specificity
The epitope in in the region of GIP (3-42).
Unit Size
150 µL: Cat. No. ABS 054-14B-015 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide
Conjugation
Biotin
Storage
2-8ºC without exposure to light. No precautions necessary during handling.
Target
Human gastric inhibitory polypeptide (GIP) is a 42 amino acid peptide belonging to the glucagon-secretin family of peptide hormones. It is secreted by endocrine cells in the duodenal mucosa and stimulates glucose-dependent insulin secretion as well as GLP-1 release from more distal endocrine (L) cells in the intestinal mucosa. GIP shows amino-acid sequence similarities to glucagon, GLP-1 and GLP-2 (from approximately 50% identity for glucagon to 30% identity for GLP-2).

ELISA: ABS 057-25 (as capture antibody) forms a sandwich ELISA pair with ABS 054-14B (as biotinylated detection antibody) for measuring GIP. Data suggest that this matched antibody pair is the most sensitive pair for measuring GIP (3-42). The detection limit obtained with our non-optimized buffer system is up to 1 ng/mL of GIP (3-42). Cross-reaction with GIP (1-42) is around 2%.

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