Mouse monoclonal antibody

Anti-Gastric Inhibitory Peptide (GIP)

CAT NO: ABS 054-24 | Application: ELISA

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Product Summary

Cat. No.

ABS 054-24

Specificity

ABS 054-24 binds human GIP (1-42).

Immunogen

Synthetic human gastric inhibitory polypeptide

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Gene ID

2695

Clone Number

Unit Size

400 µL: Cat. No. ABS 054-24-04 1 mL: Cat. No. ABS 054-24-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

2-8ºC without exposure to light. No precautions necessary during handling.

Target

Human gastric inhibitory polypeptide (GIP) is a 42 amino acid peptide belonging to the glucagon-secretin family of peptide hormones. It is secreted by endocrine cells in the duodenal mucosa and stimulates glucose-dependent insulin secretion as well as GLP-1 release from more distal endocrine (L) cells in the intestinal mucosa. GIP shows amino-acid sequence similarities to glucagon, GLP-1 and GLP-2 (from approximately 50% identity for glucagon to 30% identity for GLP-2).

Application

ELISA: ABS 054-26 (as capture antibody) forms a sandwich ELISA pair with ABS 054-24B (as a biotinylated detection antibody) for measuring GIP. The detection limit obtained with our non-optimized buffer system is up to 4 ng/mL of GIP (1-42). This pair seems to be also more sensitive then pair ABS 021-04 / ABS 054-24B with our non-optimized buffer system.

ABS 021-04 (as a capture antibody) forms a sandwich ELISA pair with ABS 054-24B (as a biotinylated detection antibody) for measuring GIP. The detection limit obtained with our non-optimized buffer system is up to 4 ng/mL of GIP (1-42).