Mouse monoclonal antibody

Anti-Glycoprotein-340 (gp-340)

CAT NO: HYB 213-06 | Application: ELISA, WB, IHC

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Product Summary

Cat. No.
HYB 213-06
Specificity
HYB 213-06 reacts with gp-340 also known as salivary scavenger and agglutinin.
Immunogen
Native gp-340 purified from bronchoalveolar lavage fluid from patients with pulmonary alveolar proteinosis.
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
1755
Clone Number
1G4
Epitope Specificity
Protein chain (1)
Unit Size
400 µL: Cat. No. HYB 213-06-04 1 mL: Cat. No. HYB 213-06-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Glycoprotein (gp)-340 is member of the scavenger receptor superfamily and has been identified as a putative SP-D receptor. Gp-340 has been shown to bind SP-D in a calcium-dependent manner through a protein-protein interaction. Like SP-D, gp-340 is found in the lungs and immunohistochemistry shows high expression in alveolar macrophages. An identical protein, salivary agglutinin, is present in saliva (1, 2, 3) and both gp-340 and salivary agglutinin are encoded by the gene DMBT1.

Application

ELISA: HYB 213-06 can be used in ELISA. (2)

WB: In Western blotting after SDS-PAGE HYB 213-06 reacts strongly with GP-340 in non-reduced forms only. A dilution guideline of 1/2000 has proved successful. (1, 2, 3, 4)

IHC: HYB 213-06 can be used in IHC. (1, 2)

Scientific References

1. Bikker FJ, Ligtenberg AJ, van der Wal JE, van den Keijbus PA, Holmskov U, Veerman EC, Nieuw Amerongen AV (2002) Immunohistochemical detection of salivary agglutinin/gp-340 in human parotid, submandibular, and labial salivary glands. J Dent Res 81:134-139.

2. Ligtenberg TJM, Bikker FJ, Groenink J, Tornoe I, Leth-Larsen R, Veerman EC, Nieuw Amerongen AV, Holmskov U (2001) Human salivary agglutinin binds to lung surfactant protein-D and is identical with scavenger receptor protein gp-340. Biochem J 359:243-248.

3. Prakobphol A, Xu F, Hoang VM, Larsson T, Bergstrom J, Johansson I, Frangsmyr L, Holmskov U, Leffler H, Nilsson C, Boren T, Wright JR, Stromberg N, Fisher SJ (2000) Salivary agglutinin, which binds Streptococcus mutans and Helicobacter pylori, is the lung scavenger receptor cysteine-rich protein gp-340. J Biol Chem 275:39860-39866.

4. Heo SM, Choi KS, Kazim LA, Reddy MS, Haase EM, Scannapieco FA, Ruhl S. (2013) Host defense proteins derived from human saliva bind to Staphylococcus aureus Infect. Immunol. 1364-73. doi: 10.1128/IAI.00825-12.

Cat. No.
HYB 213-06
Specificity
HYB 213-06 reacts with gp-340 also known as salivary scavenger and agglutinin.
Immunogen
Native gp-340 purified from bronchoalveolar lavage fluid from patients with pulmonary alveolar proteinosis.
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
1755
Clone Number
1G4
Epitope Specificity
Protein chain (1)
Unit Size
400 µL: Cat. No. HYB 213-06-04 1 mL: Cat. No. HYB 213-06-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Glycoprotein (gp)-340 is member of the scavenger receptor superfamily and has been identified as a putative SP-D receptor. Gp-340 has been shown to bind SP-D in a calcium-dependent manner through a protein-protein interaction. Like SP-D, gp-340 is found in the lungs and immunohistochemistry shows high expression in alveolar macrophages. An identical protein, salivary agglutinin, is present in saliva (1, 2, 3) and both gp-340 and salivary agglutinin are encoded by the gene DMBT1.

ELISA: HYB 213-06 can be used in ELISA. (2)

WB: In Western blotting after SDS-PAGE HYB 213-06 reacts strongly with GP-340 in non-reduced forms only. A dilution guideline of 1/2000 has proved successful. (1, 2, 3, 4)

IHC: HYB 213-06 can be used in IHC. (1, 2)

1. Bikker FJ, Ligtenberg AJ, van der Wal JE, van den Keijbus PA, Holmskov U, Veerman EC, Nieuw Amerongen AV (2002) Immunohistochemical detection of salivary agglutinin/gp-340 in human parotid, submandibular, and labial salivary glands. J Dent Res 81:134-139.

2. Ligtenberg TJM, Bikker FJ, Groenink J, Tornoe I, Leth-Larsen R, Veerman EC, Nieuw Amerongen AV, Holmskov U (2001) Human salivary agglutinin binds to lung surfactant protein-D and is identical with scavenger receptor protein gp-340. Biochem J 359:243-248.

3. Prakobphol A, Xu F, Hoang VM, Larsson T, Bergstrom J, Johansson I, Frangsmyr L, Holmskov U, Leffler H, Nilsson C, Boren T, Wright JR, Stromberg N, Fisher SJ (2000) Salivary agglutinin, which binds Streptococcus mutans and Helicobacter pylori, is the lung scavenger receptor cysteine-rich protein gp-340. J Biol Chem 275:39860-39866.

4. Heo SM, Choi KS, Kazim LA, Reddy MS, Haase EM, Scannapieco FA, Ruhl S. (2013) Host defense proteins derived from human saliva bind to Staphylococcus aureus Infect. Immunol. 1364-73. doi: 10.1128/IAI.00825-12.

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