Mouse monoclonal antibody

Anti-Neuropeptide Y (human, rat, mouse)

CAT NO: ABS 028-08 | Application: ELISA, IHC-P, IF

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Product Summary

Cat. No.
ABS 028-08
Specificity
ABS 028-08 binds full length human/rat NPY. Cross-reactivity with NPY (1-24), NPY (18-36), PYY or PP was <0.2%.
Immunogen
Synthetic human/rat neuropeptide Y
Host
Mouse
Positive Species Reactivity
Human, Rat, Mouse (murine)
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
4852, 24604
Clone Number
8
Epitope Specificity
The epitope of ABS 028-08 is probably around the mid-molecular region, NPY (18-24).
Unit Size
400 µL: Cat. No. ABS 028-08-04 1 mL: Cat. No. ABS 028-08-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Neuropeptide Y (NPY) is a 36 amino acid residue peptide with sequence and 3-D structural homology with PYY and pancreatic polypeptide (PP). NPY serves as a neurotransmitter and is exclusively localized in neurons in the central and peripheral nervous system. NPY is associated with the regulation of heart rate and food intake in several mammalian species.

Application

ELISA: ABS 028-08 binds free NPY in solution and ABS 028-08 reacts in ELISA directly with NPY coated directly onto the microtiter well.

IHC-P: ABS 028-08 works well in IHC on paraffin embedded tissue sections.

Paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) from a mouse suffering an epiletic attack just before perfusion stained with ABS 028-08 diluted 1/1000.

Enlarged section of a paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) from a mouse suffering an epileptic attack just before perfusion stained with ABS 028-08 diluted 1/1000.

Paraffin embedded mouse brain tissue (interneurons in the cortex) stained with ABS 028-08 diluted 1/1000.

IF: ABS 028-08 works well in IF.

ABS 028-08 tested on tissue from human cerebral cortex, applying ultra thin sections using a technique called array tomography. (antibody: green, DAPI-stained nuclei: blue). The antibody labels NPY-positive processes in the neutrophil. Courtesy of Dr. Nancy O'Rourke, Stanford University.

ABS 028-08 tested on tissue from human cerebral cortex, applying ultra thin sections using a technique called array tomography. (antibody: green, DAPI-stained nuclei: blue). The antibody labels NPY-positive processes in the neutrophil. Courtesy of Dr. Nancy O'Rourke, Stanford University.

Cat. No.
ABS 028-08
Specificity
ABS 028-08 binds full length human/rat NPY. Cross-reactivity with NPY (1-24), NPY (18-36), PYY or PP was <0.2%.
Immunogen
Synthetic human/rat neuropeptide Y
Host
Mouse
Positive Species Reactivity
Human, Rat, Mouse (murine)
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
4852, 24604
Clone Number
8
Epitope Specificity
The epitope of ABS 028-08 is probably around the mid-molecular region, NPY (18-24).
Unit Size
400 µL: Cat. No. ABS 028-08-04 1 mL: Cat. No. ABS 028-08-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Neuropeptide Y (NPY) is a 36 amino acid residue peptide with sequence and 3-D structural homology with PYY and pancreatic polypeptide (PP). NPY serves as a neurotransmitter and is exclusively localized in neurons in the central and peripheral nervous system. NPY is associated with the regulation of heart rate and food intake in several mammalian species.

ELISA: ABS 028-08 binds free NPY in solution and ABS 028-08 reacts in ELISA directly with NPY coated directly onto the microtiter well.

IHC-P: ABS 028-08 works well in IHC on paraffin embedded tissue sections.

Paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) from a mouse suffering an epiletic attack just before perfusion stained with ABS 028-08 diluted 1/1000.

Enlarged section of a paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) from a mouse suffering an epileptic attack just before perfusion stained with ABS 028-08 diluted 1/1000.

Paraffin embedded mouse brain tissue (interneurons in the cortex) stained with ABS 028-08 diluted 1/1000.

IF: ABS 028-08 works well in IF.

ABS 028-08 tested on tissue from human cerebral cortex, applying ultra thin sections using a technique called array tomography. (antibody: green, DAPI-stained nuclei: blue). The antibody labels NPY-positive processes in the neutrophil. Courtesy of Dr. Nancy O'Rourke, Stanford University.

ABS 028-08 tested on tissue from human cerebral cortex, applying ultra thin sections using a technique called array tomography. (antibody: green, DAPI-stained nuclei: blue). The antibody labels NPY-positive processes in the neutrophil. Courtesy of Dr. Nancy O'Rourke, Stanford University.

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