Mouse monoclonal antibody

Anti-NGAL (human)

CAT NO: ABS 062-14 | Application: ELISA

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Product Summary

Cat. No.

ABS 062-14

Specificity

ABS 062-14 is specific for human NGAL (neutrophil gelatinase-associated lipocalin).

Immunogen

Human recombinant NGAL coupled to carrier

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Isotype

IgG2a/k

Gene ID

3934

Clone Number

Epitope Specificity

Differs from those of HYB 211-01 and ABS 062-33.

Unit Size

400 µL: Cat. No. ABS 062-14-04 1 mL: Cat. No. ABS 062-14-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by acute kidney injury.

Application

ELISA: ABS 062-14 binds human NGAL coated onto a solid phase. ABS 062-14 forms a sandwich pair with HYB 211-01, ABS 038-04, -14, -15, -26 and ABS 062-33 for measuring human NGAL. When ABS 062-14 is used as a biotinylated detection antibody with ABS 062-33 as a capture antibody, the assay is specific for NGAL monomer, cross-reacting <1% with NGAL homodimer.

Scientific References

1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A.