Mouse monoclonal antibody

Anti-Placental protein 14 (human, glycodelin A)

CAT NO: BTE 001-13 | Application: ELISA, Immunoblotting, Immunohistochemistry

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Product Summary

Cat. No.
BTE 001-13
Antigen
Human placental protein 14 (PP14; also known as glycodelin and progesterone-associated endometrial protein) is a protein of the lipocalin structural superfamily. PP14 is the most abundant product of the secretory endometrium, and has been proposed as a biochemical marker of endometrial function in women undergoing in vitro fertilization treatment (1).
Immunogen
Placental protein 14 (glycodelin A) purified from second trimester amniotic fluid
Specificity
BTE 001-13 is specific for human PP14
Epitope Specificity
Differs from BTE 001-16 and -18
Isotype
IgG1/k
Reactivity
BTE 001-13 reacts strongly to human PP14 in sandwich ELISA in combination with a polyclonal antibody. When staining formalin-fixed paraffin-embedded endometrial tissue from the late secretory phase, staining is restricted to the glandular cells.
Culture Medium
RPMI 1640 with 2-10% fetal calf serum
Unit Size
400 µL: Cat. No. BTE-001-13-04 1 mL: Cat. No. BTE-001-13-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light

Application

ELISA: Usability: Yes | Dilution Guideline: 1:7000 | The dilution guideline for ELISA is based on use as a detection antibody on antigen coated directly onto the microtiter well. Users should determine the optimal dilutions for their own purposes.

Immunoblotting: Usability: Not determined

Immunohistochemistry: Usability: Yes

Scientific References

1. Westergaard LG, Wiberg N, Andersen CY, Laursen SB, Kliem A, Westergaard JG, Teisner B (1998) Circulating concentrations of placenta protein 14 during the natural menstrual cycle in women significantly reflect endometrial receptivity to implantation and pregnancy during successive assisted reproduction cycles. Hum Reprod 13:2612-2619.

2. Hustin J, Philippe E, Teisner B, Grudzinskas JG (1994) Immunohistochemical localization of two endometrial proteins in the early days of human pregnancy. Placenta 15:701-708.

3. Tornehave D, Fay TN, Teisner B, Chemnitz J, Westergaard JG, Grudzinskas JG (1989) Two fetal antigens (FA-1 and FA-2) and endometrial proteins (PP12 and PP14) isolated from amniotic fluid: localisation in the fetus and adult female genital tract. Eur J Obstet Gynecol Reprod Biol 30:221-232.

Cat. No.
BTE 001-13
Antigen
Human placental protein 14 (PP14; also known as glycodelin and progesterone-associated endometrial protein) is a protein of the lipocalin structural superfamily. PP14 is the most abundant product of the secretory endometrium, and has been proposed as a biochemical marker of endometrial function in women undergoing in vitro fertilization treatment (1).
Immunogen
Placental protein 14 (glycodelin A) purified from second trimester amniotic fluid
Specificity
BTE 001-13 is specific for human PP14
Epitope Specificity
Differs from BTE 001-16 and -18
Isotype
IgG1/k
Reactivity
BTE 001-13 reacts strongly to human PP14 in sandwich ELISA in combination with a polyclonal antibody. When staining formalin-fixed paraffin-embedded endometrial tissue from the late secretory phase, staining is restricted to the glandular cells.
Culture Medium
RPMI 1640 with 2-10% fetal calf serum
Unit Size
400 µL: Cat. No. BTE-001-13-04 1 mL: Cat. No. BTE-001-13-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light

ELISA: Usability: Yes | Dilution Guideline: 1:7000 | The dilution guideline for ELISA is based on use as a detection antibody on antigen coated directly onto the microtiter well. Users should determine the optimal dilutions for their own purposes.

Immunoblotting: Usability: Not determined

Immunohistochemistry: Usability: Yes

1. Westergaard LG, Wiberg N, Andersen CY, Laursen SB, Kliem A, Westergaard JG, Teisner B (1998) Circulating concentrations of placenta protein 14 during the natural menstrual cycle in women significantly reflect endometrial receptivity to implantation and pregnancy during successive assisted reproduction cycles. Hum Reprod 13:2612-2619.

2. Hustin J, Philippe E, Teisner B, Grudzinskas JG (1994) Immunohistochemical localization of two endometrial proteins in the early days of human pregnancy. Placenta 15:701-708.

3. Tornehave D, Fay TN, Teisner B, Chemnitz J, Westergaard JG, Grudzinskas JG (1989) Two fetal antigens (FA-1 and FA-2) and endometrial proteins (PP12 and PP14) isolated from amniotic fluid: localisation in the fetus and adult female genital tract. Eur J Obstet Gynecol Reprod Biol 30:221-232.

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