Mouse monoclonal antibody
Anti-Surfactant protein A (human)
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Product Summary
Application
ELISA: HYB 238-04 reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well.
WB: In Western blotting after SDS-PAGE HYB 238-04 reacts with reduced forms of SP-A and weaker with non-reduced forms. A dilution guideline of 1/1000 has proved successful. (2)
IHC: In immunohistochemical staining from paraffin embedded tissue samples, HYB 238-04 reacts specifically with human SP-A. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from the pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. (1, 2)
Scientific References
1. Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59.
2. Madsen J, Tornoe I, Nielsen O, Koch C, Steinhilber W, Holmskov U (2003) Expression and localization of lung surfactant protein A in human tissues. Am J Respir Cell Mol Biol 29:591-7.
ELISA: HYB 238-04 reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well.
WB: In Western blotting after SDS-PAGE HYB 238-04 reacts with reduced forms of SP-A and weaker with non-reduced forms. A dilution guideline of 1/1000 has proved successful. (2)
IHC: In immunohistochemical staining from paraffin embedded tissue samples, HYB 238-04 reacts specifically with human SP-A. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from the pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. (1, 2)
1. Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59.
2. Madsen J, Tornoe I, Nielsen O, Koch C, Steinhilber W, Holmskov U (2003) Expression and localization of lung surfactant protein A in human tissues. Am J Respir Cell Mol Biol 29:591-7.