Product Summary

Cat. No.
HYB 238-04
Specificity
HYB 238-04 is specific for human pulmonary Surfactant protein A (SP-A).
Immunogen
Native SP-A purified from bronchioaveolar lavage from proteinosis patients
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
653509
Clone Number
6F10
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. HYB 238-04-04 1 mL: Cat. No. HYB 238-04-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 45 ng/ml in healthy individuals. (1)

Application

ELISA: HYB 238-04 reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well.

WB: In Western blotting after SDS-PAGE HYB 238-04 reacts with reduced forms of SP-A and weaker with non-reduced forms. A dilution guideline of 1/1000 has proved successful. (2)

Paraffin-embedded sections of human lung immunostained with HYB 238-04. (1)

IHC: In immunohistochemical staining from paraffin embedded tissue samples, HYB 238-04 reacts specifically with human SP-A. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from the pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. (1, 2)

Scientific References

1. Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59.

2. Madsen J, Tornoe I, Nielsen O, Koch C, Steinhilber W, Holmskov U (2003) Expression and localization of lung surfactant protein A in human tissues. Am J Respir Cell Mol Biol 29:591-7.

Cat. No.
HYB 238-04
Specificity
HYB 238-04 is specific for human pulmonary Surfactant protein A (SP-A).
Immunogen
Native SP-A purified from bronchioaveolar lavage from proteinosis patients
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
653509
Clone Number
6F10
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. HYB 238-04-04 1 mL: Cat. No. HYB 238-04-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 45 ng/ml in healthy individuals. (1)

ELISA: HYB 238-04 reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well.

WB: In Western blotting after SDS-PAGE HYB 238-04 reacts with reduced forms of SP-A and weaker with non-reduced forms. A dilution guideline of 1/1000 has proved successful. (2)

Paraffin-embedded sections of human lung immunostained with HYB 238-04. (1)

IHC: In immunohistochemical staining from paraffin embedded tissue samples, HYB 238-04 reacts specifically with human SP-A. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from the pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. (1, 2)

1. Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59.

2. Madsen J, Tornoe I, Nielsen O, Koch C, Steinhilber W, Holmskov U (2003) Expression and localization of lung surfactant protein A in human tissues. Am J Respir Cell Mol Biol 29:591-7.

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