Product Summary

Cat. No.
HYB 245-02
Specificity
HYB 245-02 is specific for human SP-D.
Immunogen
Recombinant neck CRD SP-D
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
6441
Clone Number
16C11
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. HYB 245-02-04 1 mL: Cat. No. HYB 245-02-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 88 ng/ml in healthy individuals.

Application

ELISA: HYB 245-02 can be used in ELISA.

Paraffin-embedded sections of human lung immunostained with HYB 245-02. (1)

WB: In Western blotting after SDS-PAGE HYB 245-02 reacts strongly with SP-D, both in reduced and non-reduced forms. HYB 245-02 only reacts with SP-D in the presence of Ca++. A dilution guideline of 1/2000 has proved successful.

Paraffin-embedded sections of human lung immunostained with HYB 245-02. (1)

IHC: HYB 245-02 can be used in Immunohistochemistry. (1)

Scientific References

1. Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59.

Cat. No.
HYB 245-02
Specificity
HYB 245-02 is specific for human SP-D.
Immunogen
Recombinant neck CRD SP-D
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
6441
Clone Number
16C11
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. HYB 245-02-04 1 mL: Cat. No. HYB 245-02-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 88 ng/ml in healthy individuals.

ELISA: HYB 245-02 can be used in ELISA.

Paraffin-embedded sections of human lung immunostained with HYB 245-02. (1)

WB: In Western blotting after SDS-PAGE HYB 245-02 reacts strongly with SP-D, both in reduced and non-reduced forms. HYB 245-02 only reacts with SP-D in the presence of Ca++. A dilution guideline of 1/2000 has proved successful.

Paraffin-embedded sections of human lung immunostained with HYB 245-02. (1)

IHC: HYB 245-02 can be used in Immunohistochemistry. (1)

1. Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59.

×