Product Summary

Cat. No.
HYB 246-04
Specificity
HYB 246-04 is specific for SP-D from amniotic fluid, serum and bronchioalveolar lavage. HYB 246-04 does not influence SP-D’s binding to carbohydrates, but block binding to GP-340.
Immunogen
Native human SP-D purified from late pregnancy amniotic fluid
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG2a/k
Gene ID
6441
Clone Number
10B2
Epitope Specificity
Epitope is localised within the C-terminal neck CRD (carbohydrate recognition domain) of SP-D
Unit Size
400 µL: Cat. No. HYB 246-04-04 1 mL: Cat. No. HYB 246-04-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is approximately 700 ng/ml in healthy individuals. (1)

Application

ELISA: HYB 246-04 can be used in ELISA. (1, 2, 3)

WB: HYB 246-04 can be used in Western blotting. (1)

Scientific References

1. Leth-Larsen R, Nordenbaek C, Tornoe I, Moeller V, Schlosser A, Koch C, Teisner B, Junker P, Holmskov U (2003) Surfactant protein D (SP-D) serum levels in patients with community-acquired pneumonia. Clinical Immunology 108:29-37.

2. Leth-Larsen R, Garred P, Jensenius H, Meschi J, Hartshorn K, Madsen J, Tornoe I, Madsen OH, Sørensen G, Crouch E, Holmskov U (2005) A common Polymorphism in the SFTPD Gene Influences Assembly, Function, and Concentration of Surfactant Protein D. J Immunol 174:1532-1538.

3. Sorensen GL, Hoegh SV, Leth-Larsen R, Thomsen TH, Floridon C, Smith K, Kejling K, Tornoe I, Crouch EC, Holmskov U (2009) Multimeric and trimeric subunit SP-D are interconvertible structures with distinct ligand interaction. Mol Immunol 46:3060-3069.

Cat. No.
HYB 246-04
Specificity
HYB 246-04 is specific for SP-D from amniotic fluid, serum and bronchioalveolar lavage. HYB 246-04 does not influence SP-D’s binding to carbohydrates, but block binding to GP-340.
Immunogen
Native human SP-D purified from late pregnancy amniotic fluid
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG2a/k
Gene ID
6441
Clone Number
10B2
Epitope Specificity
Epitope is localised within the C-terminal neck CRD (carbohydrate recognition domain) of SP-D
Unit Size
400 µL: Cat. No. HYB 246-04-04 1 mL: Cat. No. HYB 246-04-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is approximately 700 ng/ml in healthy individuals. (1)

ELISA: HYB 246-04 can be used in ELISA. (1, 2, 3)

WB: HYB 246-04 can be used in Western blotting. (1)

1. Leth-Larsen R, Nordenbaek C, Tornoe I, Moeller V, Schlosser A, Koch C, Teisner B, Junker P, Holmskov U (2003) Surfactant protein D (SP-D) serum levels in patients with community-acquired pneumonia. Clinical Immunology 108:29-37.

2. Leth-Larsen R, Garred P, Jensenius H, Meschi J, Hartshorn K, Madsen J, Tornoe I, Madsen OH, Sørensen G, Crouch E, Holmskov U (2005) A common Polymorphism in the SFTPD Gene Influences Assembly, Function, and Concentration of Surfactant Protein D. J Immunol 174:1532-1538.

3. Sorensen GL, Hoegh SV, Leth-Larsen R, Thomsen TH, Floridon C, Smith K, Kejling K, Tornoe I, Crouch EC, Holmskov U (2009) Multimeric and trimeric subunit SP-D are interconvertible structures with distinct ligand interaction. Mol Immunol 46:3060-3069.

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