Mouse monoclonal antibody
Anti-Surfactant protein D (human, hSP-D)
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Product Summary
Application
ELISA: HYB 245-01 can be used for ELISA with Ca++ present in the buffer.
WB: In Western blotting after SDS-PAGE HYB 245-01 reacts strongly with SP-D, both in reduced and non-reduced forms. In Western blotting a dilution guideline of 1/20.000 has proved successful. (2, 3)
IHC: In immunohistochemical staining on paraffin embedded tissues, HYB 245-01 reacts specifically with SP-D. Immunoreactivity can be found in epithelial cells of the lung, skin, small intestine, esophagus, gall-bladder, kidney and urinary bladder. (1, 2, 3)
Scientific References
1. Holmskov U, Mollenhauer J, Madsen J, Vitved L, Gronlund J, Tornoe I, Kliem A, Reid KB, Poustka A, Skjodt K (1999) Cloning of GP-340, a putative opsonin receptor for lung surfactant protein D. Proc Natl Acad Sci USA 96:10794-9.
2. Madsen J, Kliem A, Tornoe I, Skjodt K, Koch C, Holmskov U (2000) Localization of lung surfactant protein D on mucosal surfaces in human tissues. J Immunol 164:5866–70.
3. Madsen J, Tornoe I, Nielsen O, Koch C, Steinhilber W, Holmskov U (2003) Expression and localization of lung surfactant protein A in human tissues. Am J Respir Cell Mol Biol 29:591-7.
ELISA: HYB 245-01 can be used for ELISA with Ca++ present in the buffer.
WB: In Western blotting after SDS-PAGE HYB 245-01 reacts strongly with SP-D, both in reduced and non-reduced forms. In Western blotting a dilution guideline of 1/20.000 has proved successful. (2, 3)
IHC: In immunohistochemical staining on paraffin embedded tissues, HYB 245-01 reacts specifically with SP-D. Immunoreactivity can be found in epithelial cells of the lung, skin, small intestine, esophagus, gall-bladder, kidney and urinary bladder. (1, 2, 3)
1. Holmskov U, Mollenhauer J, Madsen J, Vitved L, Gronlund J, Tornoe I, Kliem A, Reid KB, Poustka A, Skjodt K (1999) Cloning of GP-340, a putative opsonin receptor for lung surfactant protein D. Proc Natl Acad Sci USA 96:10794-9.
2. Madsen J, Kliem A, Tornoe I, Skjodt K, Koch C, Holmskov U (2000) Localization of lung surfactant protein D on mucosal surfaces in human tissues. J Immunol 164:5866–70.
3. Madsen J, Tornoe I, Nielsen O, Koch C, Steinhilber W, Holmskov U (2003) Expression and localization of lung surfactant protein A in human tissues. Am J Respir Cell Mol Biol 29:591-7.
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