Product Summary

Cat. No.
HYB 245-01
Specificity
HYB 245-01 is specific for human SP-D.
Immunogen
Recombinant neck CRD SP-D
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
6441
Clone Number
12G5
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. HYB 245-01-04 1 mL: Cat. No. HYB 245-01-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 88 ng/ml in healthy individuals. (1)

Application

ELISA: HYB 245-01 can be used for ELISA with Ca++ present in the buffer.

WB: In Western blotting after SDS-PAGE HYB 245-01 reacts strongly with SP-D, both in reduced and non-reduced forms. In Western blotting a dilution guideline of 1/20.000 has proved successful. (2, 3)

IHC: In immunohistochemical staining on paraffin embedded tissues, HYB 245-01 reacts specifically with SP-D. Immunoreactivity can be found in epithelial cells of the lung, skin, small intestine, esophagus, gall-bladder, kidney and urinary bladder. (1, 2, 3)

Scientific References

1. Holmskov U, Mollenhauer J, Madsen J, Vitved L, Gronlund J, Tornoe I, Kliem A, Reid KB, Poustka A, Skjodt K (1999) Cloning of GP-340, a putative opsonin receptor for lung surfactant protein D. Proc Natl Acad Sci USA 96:10794-9.

2. Madsen J, Kliem A, Tornoe I, Skjodt K, Koch C, Holmskov U (2000) Localization of lung surfactant protein D on mucosal surfaces in human tissues. J Immunol 164:5866–70.

3. Madsen J, Tornoe I, Nielsen O, Koch C, Steinhilber W, Holmskov U (2003) Expression and localization of lung surfactant protein A in human tissues. Am J Respir Cell Mol Biol 29:591-7.

Cat. No.
HYB 245-01
Specificity
HYB 245-01 is specific for human SP-D.
Immunogen
Recombinant neck CRD SP-D
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
6441
Clone Number
12G5
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. HYB 245-01-04 1 mL: Cat. No. HYB 245-01-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 88 ng/ml in healthy individuals. (1)

ELISA: HYB 245-01 can be used for ELISA with Ca++ present in the buffer.

WB: In Western blotting after SDS-PAGE HYB 245-01 reacts strongly with SP-D, both in reduced and non-reduced forms. In Western blotting a dilution guideline of 1/20.000 has proved successful. (2, 3)

IHC: In immunohistochemical staining on paraffin embedded tissues, HYB 245-01 reacts specifically with SP-D. Immunoreactivity can be found in epithelial cells of the lung, skin, small intestine, esophagus, gall-bladder, kidney and urinary bladder. (1, 2, 3)

1. Holmskov U, Mollenhauer J, Madsen J, Vitved L, Gronlund J, Tornoe I, Kliem A, Reid KB, Poustka A, Skjodt K (1999) Cloning of GP-340, a putative opsonin receptor for lung surfactant protein D. Proc Natl Acad Sci USA 96:10794-9.

2. Madsen J, Kliem A, Tornoe I, Skjodt K, Koch C, Holmskov U (2000) Localization of lung surfactant protein D on mucosal surfaces in human tissues. J Immunol 164:5866–70.

3. Madsen J, Tornoe I, Nielsen O, Koch C, Steinhilber W, Holmskov U (2003) Expression and localization of lung surfactant protein A in human tissues. Am J Respir Cell Mol Biol 29:591-7.

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