Mouse monoclonal antibody

Anti-Type III Collagen (human, dog, rat, kangaroo)

CAT NO: CSI 007-01 | Application: ELISA, WB, IHC-F

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Product Summary

Cat. No.
CSI 007-01
Specificity
CSI 007-01 is highly specific for type III collagen. It has been shown to have no cross-ractivity with type I and V collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
Immunogen
Acid-digested pepsin soluble human type III collagen
Host
Mouse
Positive Species Reactivity
Human, Rat, Dog, Kangaroo
Negative Species Reactivity
Calf, Sheep
Isotype
IgG1/k
Gene ID
1281, 84032, 478835,
Clone Number
1E7-D7/Col3
Epitope Specificity
Epitope is located approximately 100nm from the N-terminal of the molecule
Unit Size
400 µL: Cat. No. CSI 007-01-04 1 mL: Cat. No. CSI 007-01-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Type III collagen is present in the walls of arteries, intestines and the uterus, where it strenghtens the connective tissue. Patients with defects in the type III collagen (ex. Ehlers-Danlos syndrome) are at risk of rupture of major arteries or the intestine.

Application

ELISA: CSI 007-01 can be used for detection of collagens by ELISA.

WB: In immunoblotting CSI 007-01 detects type III collagen only in its native triple helix form.

IHC-F: CSI 007-01 has been used successfully for immunohistology on frozen unfixed sections of human (1) and kangaroo (2) skin, and of new dog tissue associated with biomaterial implants. (3, 4) If fixation of tissue is required, acetone or ethanol is recommended. (1, 2, 3, 4) Please consult www.proteinatlas.org

Paraffin-embedded human bronchus tissue immunostained with CSI 007-01. (www.proteinatlas.org)

Scientific References

1. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402.

2. Stephens LJ, Werkmeister JA, Tebb TA, Ramshaw JAM (1991) Identification of type III collagen from kangaroo skin. Das Leder 42:41-44.

3. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.

4. Werkmeister JA, Glattauer V, Tebb TA, Ramshaw JAM, Edwards GA, Robets G (1991) Structural stability of long-term implants of a collagen-based vascular prosthesis. J Long Term Eff Med Implants 1:107-119

Cat. No.
CSI 007-01
Specificity
CSI 007-01 is highly specific for type III collagen. It has been shown to have no cross-ractivity with type I and V collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
Immunogen
Acid-digested pepsin soluble human type III collagen
Host
Mouse
Positive Species Reactivity
Human, Rat, Dog, Kangaroo
Negative Species Reactivity
Calf, Sheep
Isotype
IgG1/k
Gene ID
1281, 84032, 478835,
Clone Number
1E7-D7/Col3
Epitope Specificity
Epitope is located approximately 100nm from the N-terminal of the molecule
Unit Size
400 µL: Cat. No. CSI 007-01-04 1 mL: Cat. No. CSI 007-01-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Type III collagen is present in the walls of arteries, intestines and the uterus, where it strenghtens the connective tissue. Patients with defects in the type III collagen (ex. Ehlers-Danlos syndrome) are at risk of rupture of major arteries or the intestine.

ELISA: CSI 007-01 can be used for detection of collagens by ELISA.

WB: In immunoblotting CSI 007-01 detects type III collagen only in its native triple helix form.

IHC-F: CSI 007-01 has been used successfully for immunohistology on frozen unfixed sections of human (1) and kangaroo (2) skin, and of new dog tissue associated with biomaterial implants. (3, 4) If fixation of tissue is required, acetone or ethanol is recommended. (1, 2, 3, 4) Please consult www.proteinatlas.org

Paraffin-embedded human bronchus tissue immunostained with CSI 007-01. (www.proteinatlas.org)

1. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402.

2. Stephens LJ, Werkmeister JA, Tebb TA, Ramshaw JAM (1991) Identification of type III collagen from kangaroo skin. Das Leder 42:41-44.

3. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.

4. Werkmeister JA, Glattauer V, Tebb TA, Ramshaw JAM, Edwards GA, Robets G (1991) Structural stability of long-term implants of a collagen-based vascular prosthesis. J Long Term Eff Med Implants 1:107-119

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Non-hazardous statement (SDS)

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