Mouse monoclonal antibody

Anti-yENaC (y−subunit epithelial sodium channel)

CAT NO: CAM 003-07 | Application: ELISA, WB, IHC-P, IHC-F

Request Price

"*" indicates required fields

Name*

First Last

Email*

Organization

Shipping Address*

City, State, Post Code/Zip*

Country*

Phone

Catalog Number ( Product )

Message

Phone

This field is for validation purposes and should be left unchanged.

Product Summary

Cat. No.

CAM 003-07

Specificity

CAM 003-07 is specific for the inhibitory tract of human yENaC subunit.

Immunogen

The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human yENaC subunit.

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Clone Number

3c7

Epitope Specificity

The epitope is on the inhibitory tract of human ENaC. The epitope differs from that of CAM 005-02.

Unit Size

400 µL: Cat. No. CAM 003-07-04 1 mL: Cat. No. CAM 003-07-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the y-subunit and putative release of a 43-amino acid inhibitory tract from the y-subunit ectodomain.

Application

ELISA: CAM 003-07 was used in ELISA. A sandwich ELISA can be made using CAM 003-07 (1-4 μg/ml) as the capture antibody and biotinylated CAM 005-02 (0.05 - 0.2 μg/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131).

WB: CAM 003-07 was used in Western blot. (1,2)

WB performed with CAM 003-07 on a lysate of HEK293 cells transfected with and without γENaC. Lane 1 loaded with 5µg, Lane 2 loaded with 10µg, Lane 3 loaded with 20 µg.

IHC-P: CAM 003-07 was used in immunohistochemistry.

IHC-F: CAM 003-07 was used in immunohistochemistry.

Scientific References

1. Svenningsen P, Uhrenholt TR, Palarasah Y, Skjodt K, Jensen BL, Skott O (2009) Prostasin-dependent activation of epithelial Na+ channels by low plasmin concentrations. Am J Physiol Regul Integr Comp Physiol 297:R1733-R1741.

2. Zachar RM, Skjødt K, Marcussen N, Walter S, Toft A, Nielsen MR, Jensen BL, Svenningsen P. The Epithelial Sodium Channel-Subunit Is Processed Proteolytically in Human Kidney. J Am Soc Nephrol. 2014 Jul 24. pii: ASN.2013111173. (epub ahead of print).