Mouse monoclonal antibody

Anti-Aprotinin

CAT NO: ABS 037-18 | Application: ELISA, WB

Request Price

"*" indicates required fields

Name*

First Last

Email*

Organization

Shipping Address*

City, State, Post Code/Zip*

Country*

Phone

Catalog Number ( Product )

Message

Phone

This field is for validation purposes and should be left unchanged.

Product Summary

Cat. No.

ABS 037-18

Specificity

ABS 037-18 binds bovine aprotinin immobilized on a solid phase or free in solution.

Immunogen

Aprotinin from bovine lung.

Host

Mouse

Positive Species Reactivity

Bovine

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

404172

Clone Number

Epitope Specificity

The epitope of ABS 037-18 is different from the epitopes of ABS 037-21 and ABS 037-35.

Unit Size

400 µL: Cat. No. ABS 037-18-04 1 mL: Cat. No. ABS 037-18-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury.

Application

ELISA: ABS 037-18 (as biotinylated detection antibody e.g. at 1 μg/mL) forms an antibody pair with ABS 037-21 (as capture antibody) for high-sensitivity sandwich ELISA of aprotinin at sample concentrations of 0.6-20 μg/mL. Aprotinin in urine diluted 1/1000 can be assayed against standards in buffer. For assay in plasma it is recommended to add aprotinin-free control plasma to the standards to match the plasma sample dilution.

ABS 037-18 also forms an antibody pair with ABS 037-35 (as capture antibody) for sandwich ELISA of aprotinin at sample concentrations of 4-250 μg/mL. Both urine and plasma samples diluted 1/1000 can be assayed against standards in buffer. PBS-buffers are unsuitable for these assays and 0.2 M glycine sodium buffer, pH 8.8, is recommended.

WB: ABS 037-18 can be used in Western blotting.