Mouse monoclonal antibody

Anti-Aprotinin

CAT NO: ABS 037-18 | Application: ELISA, WB

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Product Summary

Cat. No.
ABS 037-18
Specificity
ABS 037-18 binds bovine aprotinin immobilized on a solid phase or free in solution.
Immunogen
Aprotinin from bovine lung.
Host
Mouse
Positive Species Reactivity
Bovine
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
404172
Clone Number
18
Epitope Specificity
The epitope of ABS 037-18 is different from the epitopes of ABS 037-21 and ABS 037-35.
Unit Size
400 µL: Cat. No. ABS 037-18-04 1 mL: Cat. No. ABS 037-18-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury.

Application

ELISA: ABS 037-18 (as biotinylated detection antibody e.g. at 1 μg/mL) forms an antibody pair with ABS 037-21 (as capture antibody) for high-sensitivity sandwich ELISA of aprotinin at sample concentrations of 0.6-20 μg/mL. Aprotinin in urine diluted 1/1000 can be assayed against standards in buffer. For assay in plasma it is recommended to add aprotinin-free control plasma to the standards to match the plasma sample dilution.

ABS 037-18 also forms an antibody pair with ABS 037-35 (as capture antibody) for sandwich ELISA of aprotinin at sample concentrations of 4-250 μg/mL. Both urine and plasma samples diluted 1/1000 can be assayed against standards in buffer. PBS-buffers are unsuitable for these assays and 0.2 M glycine sodium buffer, pH 8.8, is recommended.

WB: ABS 037-18 can be used in Western blotting.

Cat. No.
ABS 037-18
Specificity
ABS 037-18 binds bovine aprotinin immobilized on a solid phase or free in solution.
Immunogen
Aprotinin from bovine lung.
Host
Mouse
Positive Species Reactivity
Bovine
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
404172
Clone Number
18
Epitope Specificity
The epitope of ABS 037-18 is different from the epitopes of ABS 037-21 and ABS 037-35.
Unit Size
400 µL: Cat. No. ABS 037-18-04 1 mL: Cat. No. ABS 037-18-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury.

ELISA: ABS 037-18 (as biotinylated detection antibody e.g. at 1 μg/mL) forms an antibody pair with ABS 037-21 (as capture antibody) for high-sensitivity sandwich ELISA of aprotinin at sample concentrations of 0.6-20 μg/mL. Aprotinin in urine diluted 1/1000 can be assayed against standards in buffer. For assay in plasma it is recommended to add aprotinin-free control plasma to the standards to match the plasma sample dilution.

ABS 037-18 also forms an antibody pair with ABS 037-35 (as capture antibody) for sandwich ELISA of aprotinin at sample concentrations of 4-250 μg/mL. Both urine and plasma samples diluted 1/1000 can be assayed against standards in buffer. PBS-buffers are unsuitable for these assays and 0.2 M glycine sodium buffer, pH 8.8, is recommended.

WB: ABS 037-18 can be used in Western blotting.

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Product Specification

Non-hazardous statement (SDS)

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