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Product Summary
Application
ELISA: ABS 037-18 (as biotinylated detection antibody e.g. at 1 μg/mL) forms an antibody pair with ABS 037-21 (as capture antibody) for high-sensitivity sandwich ELISA of aprotinin at sample concentrations of 0.6-20 μg/mL. Aprotinin in urine diluted 1/1000 can be assayed against standards in buffer. For assay in plasma it is recommended to add aprotinin-free control plasma to the standards to match the plasma sample dilution. ABS 037-18 also forms an antibody pair with ABS 037-35 (as capture antibody) for sandwich ELISA of aprotinin at sample concentrations of 4-250 μg/mL. Both urine and plasma samples diluted 1/1000 can be assayed against standards in buffer. PBS-buffers are unsuitable for these assays and 0.2 M glycine sodium buffer, pH 8.8, is recommended.
WB: ABS 037-18 can be used in Western blotting.
ELISA: ABS 037-18 (as biotinylated detection antibody e.g. at 1 μg/mL) forms an antibody pair with ABS 037-21 (as capture antibody) for high-sensitivity sandwich ELISA of aprotinin at sample concentrations of 0.6-20 μg/mL. Aprotinin in urine diluted 1/1000 can be assayed against standards in buffer. For assay in plasma it is recommended to add aprotinin-free control plasma to the standards to match the plasma sample dilution. ABS 037-18 also forms an antibody pair with ABS 037-35 (as capture antibody) for sandwich ELISA of aprotinin at sample concentrations of 4-250 μg/mL. Both urine and plasma samples diluted 1/1000 can be assayed against standards in buffer. PBS-buffers are unsuitable for these assays and 0.2 M glycine sodium buffer, pH 8.8, is recommended.
WB: ABS 037-18 can be used in Western blotting.
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