Mouse monoclonal antibody, biotinylated

Anti-Complement component C3a/C3a(desArg)/C3 (human)

CAT NO: GAU 017-01B | Application: ELISA, IHC

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Product Summary

Cat. No.

GAU 017-01B

Specificity

Recognizes an epitope that is present on human C3, C3a and C3a (desArg). Does not cross-react with C4a or C5a.

Immunogen

Human C3a

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

718

Clone Number

D17/1

Epitope Specificity

GAU 017-01 recognizes different epitopes on the 9 kDa C3a than GAU 013-16. No reaction is seen with a synthetic octapeptide representing the C3a C-terminal.

Unit Size

150 µL, 1 mg/mL +/- 15%. Cat. No. GAU 017-01B-015 See Certificate of Analysis for details.

Purification

Biotinylated

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide

Conjugation

Biotin

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Complement C3a is an anaphylatoxin of 77 amino acid residues released by the action of the C3 convertases on the N-terminal of the alpha chain of C3. It is rapidly inactivated by serum carboxypeptidase N which removes the C-terminal arginine residue generating C3a (desArg).

Application

ELISA: GAU 017-01 can be used as a biotinylated detection antibody in sandwich ELISA with GAU 013-16 capture antibody (1-5). Does not inhibit the biological activity of C3a. (1)

IHC: GAU 017-01B was used in IHC on human appendix section.

IHC staining of human appendix section. Plasma of veins stained strongly. Dilution: 1:30

Scientific References

1. Nezlin R, Freywald A, Oppermann M (1993) Proteins separeted from human IgG molecules. Mol. Immunol. 30:935-940.

2. Oppermann M, Liebmann F, Götze O. (1987) Purification and quantification of human C3a anaphylatoxin using monoclonal antibodies. Complement 4:205-206

3. Oppermann M, Haubitz M, Quintin E, Götze O (1988) Complement activation in patients with renal failure as detected through the quantitation of fragments of the complement proteins C3, C5, and Factor B. Klin Wochenschr 66:857-864.

4. Khodoun M, Strait R, Orekov T, Hogan S, Karasuyama H, Herbert DBR, Köhl J, Finkelman FD (2009) Peanuts can contribute to anaphylactic shock by activating complement. J Allergy Clin Immunol 123:342-351.

5. Thomas SN, van der Vlies AJ, O’Neil CP, Reddy ST, Yu SS, Giorgio TD, Swartz MA, Hubbell JA (2011) Engineering complement activation on polypropylene sulfide vaccine nanoparticles. Biomaterials 32: 2194-2203.