Mouse monoclonal antibody

Anti-D-dimer (human, dog)

CAT NO: ABS 015-22 | Application: ELISA, WB

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Product Summary

Cat. No.
ABS 015-22
Specificity
ABS 015-22 binds human D-dimer. Cross-reacts < 2% with directly coated D-monomer but not with directly coated fibrinogen.
Immunogen
Purified human D-dimer
Host
Mouse
Positive Species Reactivity
Human, Dog
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
2243, 475473
Clone Number
22
Epitope Specificity
When tested in a pair with itself, with ABS 015-28 and with a fibrinogen-specific polyclonal antibody, the epitope appears to relate to the dimenzation site of D-dimer.
Unit Size
400 µL: Cat. No. ABS 015-22-04 1 mL: Cat. No. ABS 015-22-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
D-dimer is a fibrin degradation product, a small protein fragment present in the blood after a blood clot is degraded by fibrinolysis.

Application

ELISA: ABS 015-22 binds free D-dimer in solution. ABS 015-022 works as a capture antibody in sandwich ELISA with biotinylated ABS 015-22 or ABS 015-28 as a detection antibody. Biotinylated ABS 015-22 works as a detection antibody in sandwich ELISA for D-dimer with itself (ABS 015-22) or ABS 015-28 as a capture antibody. The combination with ABS 015-28 as a capture antibody and ABS 015-22B as a detection antibody is recommended.

WB: Due to reducing conditions in Western blotting ABS 015-22 binds the alpha-chain (12kDa) of D-Dimer, and not the entire D-Dimer complex.

Cat. No.
ABS 015-22
Specificity
ABS 015-22 binds human D-dimer. Cross-reacts < 2% with directly coated D-monomer but not with directly coated fibrinogen.
Immunogen
Purified human D-dimer
Host
Mouse
Positive Species Reactivity
Human, Dog
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
2243, 475473
Clone Number
22
Epitope Specificity
When tested in a pair with itself, with ABS 015-28 and with a fibrinogen-specific polyclonal antibody, the epitope appears to relate to the dimenzation site of D-dimer.
Unit Size
400 µL: Cat. No. ABS 015-22-04 1 mL: Cat. No. ABS 015-22-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
D-dimer is a fibrin degradation product, a small protein fragment present in the blood after a blood clot is degraded by fibrinolysis.

ELISA: ABS 015-22 binds free D-dimer in solution. ABS 015-022 works as a capture antibody in sandwich ELISA with biotinylated ABS 015-22 or ABS 015-28 as a detection antibody. Biotinylated ABS 015-22 works as a detection antibody in sandwich ELISA for D-dimer with itself (ABS 015-22) or ABS 015-28 as a capture antibody. The combination with ABS 015-28 as a capture antibody and ABS 015-22B as a detection antibody is recommended.

WB: Due to reducing conditions in Western blotting ABS 015-22 binds the alpha-chain (12kDa) of D-Dimer, and not the entire D-Dimer complex.

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Product Specification

Non-hazardous statement (SDS)

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