Mouse monoclonal antibody

Anti-Procollagen type IIA N-terminal propeptide (human, PIIANP)

CAT NO: BTE 003-02 | Application: ELISA, IHC-P

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Product Summary

Cat. No.

BTE 003-02

Specificity

BTE 003-02 is specific for human PIIANP.

Immunogen

Recombinant expressed PIIANP

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

1280

Clone Number

Epitope Specificity

Epitope is located at the amino-terminal part of procollagen type IIA

Unit Size

400 µL: Cat. No. BTE 003-02-04 1 mL: Cat. No. BTE 003-02-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Collagen type II is the most abundant protein of cartilage matrix. Two isoforms exist and collagen type IIA is involved in early development of cartilage in embryos. The collagen is produced from type II procollagen when two teloterminal propeptides are digested from the procursor and released into the circulation. Procollagen type II N-terminal propeptide (PIIANP) is considered a marker of cartilage synthesis and the normal concentration in serum is approximately 30ng/mL.

Application

ELISA: BTE 003-02 also reacts with recombinant PIIANP coated directly onto the microtiter well.

IHC-P: BTE 003-02 diluted in 10%FCS/TBS is well suited for immunohistochemistry on formalin-fixed, paraffin-embedded fetal tissue.

Immunohistochemical staining of a formalin-fixed, paraffin-embedded fetal bone anlage in the hand (around week 10) with BTE 003-02.

Immunohistochemical staining of a formalin-fixed, paraffin-embedded biopsy from a chondroblastoma, showing specific staining of tumor cells with AntibodyShop product BTE 003-02 developed with peroxidase-labeled goat anti-mouse Ig (blue color).