Mouse monoclonal antibody

Anti-Vasoactive Intestinal Peptide (human, bovine, porcine, rat, VIP)

CAT NO: ABS 023-02 | Application: ELISA, WB, IHC, IF

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Product Summary

Cat. No.
ABS 023-02
Specificity
ABS 023-02 binds to human, bovine, porcine or rat VIP, but does not bind to PHM or PACAP.
Immunogen
Vasoactive intestinal peptide
Host
Mouse
Positive Species Reactivity
Human, Bovine, Pig, Rat
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
7432
Clone Number
2
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. ABS 023-02-04 1 mL: Cat. No. ABS 023-02-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Vasoactive intestinal peptide (VIP) is a 28 amino acid peptide belonging to the secretin-glucagon family of peptide hormones. Its sequence is identical in humans, cows, pigs and rats. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumors secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhea, hypokalemia and achlorhydria syndrome. VIP shows high sequence homology with peptide histidine-methionine (PHM) in humans or peptide histidine-serine (PHI) in pigs or rats, present in the same precursor, and with the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP).

Application

ELISA: A dilution guideline of 1/1000 applies to VIP coated directly at 2 μg/mL. For glutaraldehyde-linked VIP-ovalbumin (molar ratio 4:1) coated at 2 μg/mL, the dilution is 1/32,000.

WB: ABS 023-02 can be used in Western blotting.

IHC: ABS 023-02 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:100.

Formalin fixed paraffin-embedded human appendix tissue stained with ABS 023-02. Dilution: 1:100

Formalin fixed paraffin-embedded human appendix tissue stained with ABS 023-02. Dilution: 1:100

Formalin fixed paraffin-embedded human pancreas tissue stained with ABS 023-02. Dilution: 1:100

IF: ABS 023-02 was used in IF with a dilution of 1:100.

IF staining in human pancreas tissue stained with ABS 023-02. Signal is strong in nerves of exocrine part of pancreas. Dilution: 1:100.

Cat. No.
ABS 023-02
Specificity
ABS 023-02 binds to human, bovine, porcine or rat VIP, but does not bind to PHM or PACAP.
Immunogen
Vasoactive intestinal peptide
Host
Mouse
Positive Species Reactivity
Human, Bovine, Pig, Rat
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
7432
Clone Number
2
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. ABS 023-02-04 1 mL: Cat. No. ABS 023-02-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Vasoactive intestinal peptide (VIP) is a 28 amino acid peptide belonging to the secretin-glucagon family of peptide hormones. Its sequence is identical in humans, cows, pigs and rats. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumors secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhea, hypokalemia and achlorhydria syndrome. VIP shows high sequence homology with peptide histidine-methionine (PHM) in humans or peptide histidine-serine (PHI) in pigs or rats, present in the same precursor, and with the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP).

ELISA: A dilution guideline of 1/1000 applies to VIP coated directly at 2 μg/mL. For glutaraldehyde-linked VIP-ovalbumin (molar ratio 4:1) coated at 2 μg/mL, the dilution is 1/32,000.

WB: ABS 023-02 can be used in Western blotting.

IHC: ABS 023-02 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:100.

Formalin fixed paraffin-embedded human appendix tissue stained with ABS 023-02. Dilution: 1:100

Formalin fixed paraffin-embedded human appendix tissue stained with ABS 023-02. Dilution: 1:100

Formalin fixed paraffin-embedded human pancreas tissue stained with ABS 023-02. Dilution: 1:100

IF: ABS 023-02 was used in IF with a dilution of 1:100.

IF staining in human pancreas tissue stained with ABS 023-02. Signal is strong in nerves of exocrine part of pancreas. Dilution: 1:100.

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Non-hazardous statement (SDS)

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