Mouse monoclonal antibody

Anti-Factor XII (human, heavy chain)

CAT NO: CAM 011-37 | Application: ELISA, WB

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Product Summary

Cat. No.

CAM 011-37

Specificity

CAM 011-37 binds the heavy chain of human factor XII, including factor XII derivatives such as α-factor XIIa and α-factor XIIa C1-inhibitor complexes.

Immunogen

Affinity purified human factor XII.

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Isotype

IgG2a/k

Clone Number

10-11-37

Epitope Specificity

Differs from CAM 011-05.

Unit Size

400 µL: Cat. No. CAM 111-37-04 1 mL: Cat. No. CAM 111-37-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Factor XII is a component in the coagulation cascade. It is a plasma glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII to a-factor XIIa, which in turn activates factor XI to factor XIa.

Application

ELISA: CAM 011-37 was used in ELISA. CAM 011-37 as a capture antibody (1.6 μg/mL) was used in combination with biotinylated CAM 011-05 (1μg/mL) to measure total factor XII, including factor XII derivatives such as α-factor XIIa and α-factor XIIa C1-inhibitor complexes. (1)

WB: CAM 011-37 was used in western blot to detect factor XII, α-factor XIIa C1-inh complexes and α-factor XIIa. (1)

Scientific References

1. Madsen DE, Sidelmann J, Overgaard K, Koch C, Brodersen Gram J (2013) ELISA for determination of total coagulation factor XII concentration in human plasma. J Imm Meth 394:32–9.