A quantitative assay for  the in vitro determination of pig NGAL in urine, plasma, serum, tissue extracts or culture media.

Pig NGAL ELISA Kit

CAT NO: KIT 044

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Product Summary

Cat. No.
KIT 044
Assay range
4-400 pg/mL
Assay time
<4 hours
Limit of detection
1 pg/mL
Species reactivity (positive)
None
Storage
4°C
Target
Pig NGAL (Neutrophil Gelatinase-Associated Lipocalin)
Background
NGAL determination allows kidney injury to be detected almost as soon as it occurs, and not at the later stage when the consequences of impaired kidney function can be detected by existing routine tests and the damage has been consolidated. The pig is an often used experimental model for medical research because of its close resemblance to humans. Pigs are being used in everything from experimental treatments to experimental surgical procedures. The pharmaceutical industry is also looking more and more to pigs as a model for drug safety assessment in drug development as pigs are claimed to be more robust than the traditional dog models and in some cases a better mirror model of humans than dogs or even monkeys. The Pig NGAL ELISA Kit allows for the determination of rises in pig NGAL concentrations and hence the extent of renal injury, including nephrotoxic injury, to be determined, e.g. in the following settings: medical research, veterinary research, discovery, research and development, pre-clinical phase.
Regulatory status
RUO - Research Use Only.
Other information
Kit features highly reproducible and easy-to-use working solutions, ready-to-use calibrators and pre-coated 96-well ELISA plate. All incubations performed at room temperature. Requires only standard ELISA equipment.

Scientific References

23291730 – Matejkova S,Scheuerle A,Wagner F,McCook O,Matallo J,Groger M,Seifritz A,Stahl B,Vcelar B,Calzia E,Georgieff M,Moller P,Schelzig H,Radermacher P,Simon F (2013) Carbamylated erythropoietin-FC fusion protein and recombinant human erythropoietin during porcine kidney ischemia/reperfusion injury. Intensive Care Med. 39:497-510

22221604 – Hosgood SA, Hunter JP, Nicholson ML (2012) Early urinary biomarkers of warm and cold ischemic injury in an experimental kidney model. J Surg Res. 174:85-90

22671281 – Pedersen SS, Keller AK, Rehling M, Birn H, Jespersen B (2012) NGAL excretion is higher from the healthy side than from the injured side in unilateral acute kidney injury. Scand J Clin Lab Invest. 72:510-512

22584014 – Ravlo K, Chhoden T, Sondergaard P, Secher N, Keller AK, Pedersen M, Bibby BM, Jorgensen TM, Moldrup U, Ostraat EO, Birn H, Norregaard R, Marcussen N (2012) Early outcome in renal transplantation from large donors to small and size-matched recipients – a porcine experimental model. Pediatr Transplant. 16:599-606

22932187 – Wu RP, Liang XB, Guo H, Zhou XS, Zhao L, Wang C, Li RS (2012) Protective effect of low potassium dextran solution on acute kidney injury following acute lung injury induced by oleic acid in piglets. Chin Med J (Engl). 125:3093-3097

Cat. No.
KIT 044
Assay range
4-400 pg/mL
Assay time
<4 hours
Limit of detection
1 pg/mL
Species reactivity (positive)
None
Storage
4°C
Target
Pig NGAL (Neutrophil Gelatinase-Associated Lipocalin)
Background
NGAL determination allows kidney injury to be detected almost as soon as it occurs, and not at the later stage when the consequences of impaired kidney function can be detected by existing routine tests and the damage has been consolidated. The pig is an often used experimental model for medical research because of its close resemblance to humans. Pigs are being used in everything from experimental treatments to experimental surgical procedures. The pharmaceutical industry is also looking more and more to pigs as a model for drug safety assessment in drug development as pigs are claimed to be more robust than the traditional dog models and in some cases a better mirror model of humans than dogs or even monkeys. The Pig NGAL ELISA Kit allows for the determination of rises in pig NGAL concentrations and hence the extent of renal injury, including nephrotoxic injury, to be determined, e.g. in the following settings: medical research, veterinary research, discovery, research and development, pre-clinical phase.
Regulatory status
RUO - Research Use Only.
Other information
Kit features highly reproducible and easy-to-use working solutions, ready-to-use calibrators and pre-coated 96-well ELISA plate. All incubations performed at room temperature. Requires only standard ELISA equipment.

23291730 – Matejkova S,Scheuerle A,Wagner F,McCook O,Matallo J,Groger M,Seifritz A,Stahl B,Vcelar B,Calzia E,Georgieff M,Moller P,Schelzig H,Radermacher P,Simon F (2013) Carbamylated erythropoietin-FC fusion protein and recombinant human erythropoietin during porcine kidney ischemia/reperfusion injury. Intensive Care Med. 39:497-510

22221604 – Hosgood SA, Hunter JP, Nicholson ML (2012) Early urinary biomarkers of warm and cold ischemic injury in an experimental kidney model. J Surg Res. 174:85-90

22671281 – Pedersen SS, Keller AK, Rehling M, Birn H, Jespersen B (2012) NGAL excretion is higher from the healthy side than from the injured side in unilateral acute kidney injury. Scand J Clin Lab Invest. 72:510-512

22584014 – Ravlo K, Chhoden T, Sondergaard P, Secher N, Keller AK, Pedersen M, Bibby BM, Jorgensen TM, Moldrup U, Ostraat EO, Birn H, Norregaard R, Marcussen N (2012) Early outcome in renal transplantation from large donors to small and size-matched recipients – a porcine experimental model. Pediatr Transplant. 16:599-606

22932187 – Wu RP, Liang XB, Guo H, Zhou XS, Zhao L, Wang C, Li RS (2012) Protective effect of low potassium dextran solution on acute kidney injury following acute lung injury induced by oleic acid in piglets. Chin Med J (Engl). 125:3093-3097

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