An ELISA for the in vitro determination of human NGAL in urine, serum, plasma, tissue extracts or culture media.

NGAL ELISA Kit (human)

CAT NO: KIT 036

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Product Summary

Cat. No.

KIT 036

Assay range

10-1000 pg/mL

Assay time

4 hours

Limit of detection

4 pg/mL

Sample dilution

1/500 (Urine from healthy subjects)

Species reactivity (positive)

None

Storage

4°C

Target

Human NGAL (Neutrophil Gelatinase-Associated Lipocalin)

Background

NGAL is a new early biomarker for diagnosing acute kidney injury (AKI). Under normal conditions, NGAL levels are low in urine and plasma, but they rise sharply from basal levels in response to kidney injury to reach diagnostic levels within a very short time - as much as 24 hours or more before any significant rise in serum creatinine.

Regulatory status

RUO - Research Use Only

Patent status

This product is protected by one or more US, European and/or foreign patents.

Scientific References

24518531 – Hasse-Fielitz A, Haase M, Devarajan P (2014) Neutrophil gelatinase-associated lipocalin as a biomarker of acute kidney injury: a critical evaluation of current status. Ann Clin Biochem 51:335-351

23341719 – Cho E, Yang HN, Jo SK, Cho WY, Kim HK (2013) The role of urinary liver-type fatty acid-binding protein in critically ill patients. J Korean Med Sci. 28:100-105

23428967 – Di NM, Ficarella A, Ricci Z, Luciano R, Stoppa F, Picardo S, Picca S, Muraca M, Cogo P (2013)Impact of severe sepsis on serum and urinary biomarkers of acute kidney injury in critically ill children: an observational study. Blood Purif 35: 172-176

23388612 – Kashani K et al (2013) Discovery and validation of cell cycle arrest biomarkers in human acute kidney injury. Crit Care 17:R25

23605129 – Kift RL et al (2013) A comparison of the analytical performance of five commercially available assays for neutrophil gelatinase-associated lipocalin using urine. Ann Clin Biochem. 50: 236-244