Mouse monoclonal antibody

Anti-Complement component C5b-9 (human)

CAT NO: DIA 011-01 | Application: ELISA, WB, IHC, IF

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Product Summary

Cat. No.

DIA 011-01

Specificity

DIA 011-01 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive). (1)

Immunogen

Purified C5b-9

Host

Mouse

Positive Species Reactivity

Human, Pig, Horse, Baboon

Negative Species Reactivity

Not determined

Isotype

IgG2a/k

Gene ID

727

Clone Number

aE11

Epitope Specificity

DIA 011-01 binds to a neoepitope exposed on C9

Unit Size

400 µL: Cat. No. DIA 011-01-04 1 mL: Cat. No. DIA 011-01-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.15 M NaCl, 15mM NaN3

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and DIA 011-01 is directed against a neoepitope exposed on C9 when incorporated into the TCC.

Other Info

This product is not for further manaufacture.

Application

ELISA: DIA 011-01 is well suited for quantifying TCC in ELISA. (2,3)

WB: DIA 011-01 is not recommended for Western blotting as the epitope is destroyed during the process. (4)

IHC: TCC in various tissues by immunohistochemistry (frozen and paraffin sections). (1, 2, 4)

IHC staining of frozen tonsil section. Vascular endothelia and dendritic cells of germinal center have strong and intensive label. Dilution: 1:10

IF: DIA 011-01 can be used in immunofluorescence staining. (5)

Scientific References

1. Mollnes TE, Harboe M (1987) Immunohistochemical detection of the membrane and fluid-phase terminal complement complexes C5b-9(m) and SC5b-9. Consequences for interpretation and terminology. Scand J Immunol 26:381-6.

2. Mollnes TE, Lea T, Harboe M, Tschopp J (1985) Monoclonal antibodies recognizing a neoantigen of poly (C9) detect the human terminal complement complex in tissue and plasma. Scand J Immunol 22:183-195.

3. Palarasah,Y, Nielsen C, Sprogøe U, Christensen ML, Lillevang S, Madsen HO, Bygum A, Koch C, Skjodt K, Skjoed MO (2011) Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system. Clin Exp Immun 164: 388-395.

4. Mollnes TE (1997) Analysis of in vivo complement activation. Herzenberg LA, Weir DM, Herzenberg LA, Blackwell C: Weir’s Handbook of Experimental Immunology. Boston, MA: Blackwell Science, pp. 78.1-78.8.

5. Jansen J, Høgåsen K, Mollnes T (1993) Extensive Complement Activation in Hereditary Porcine Membranoproliferative Glomerulonephritis Type 11 (Porcine Dense Deposit Disease. Am J Path 194: 1356-1365.