Mouse monoclonal antibody
Anti-Complement component C5b-9 (human)
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Product Summary
Application
ELISA: DIA 011-01 is well suited for quantifying TCC in ELISA. (2,3)
WB: DIA 011-01 is not recommended for Western blotting as the epitope is destroyed during the process. (4)
IHC: TCC in various tissues by immunohistochemistry (frozen and paraffin sections). (1, 2, 4)
IF: DIA 011-01 can be used in immunofluorescence staining. (5)
Scientific References
1. Mollnes TE, Harboe M (1987) Immunohistochemical detection of the membrane and fluid-phase terminal complement complexes C5b-9(m) and SC5b-9. Consequences for interpretation and terminology. Scand J Immunol 26:381-6.
2. Mollnes TE, Lea T, Harboe M, Tschopp J (1985) Monoclonal antibodies recognizing a neoantigen of poly (C9) detect the human terminal complement complex in tissue and plasma. Scand J Immunol 22:183-195.
3. Palarasah,Y, Nielsen C, Sprogøe U, Christensen ML, Lillevang S, Madsen HO, Bygum A, Koch C, Skjodt K, Skjoed MO (2011) Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system. Clin Exp Immun 164: 388-395.
4. Mollnes TE (1997) Analysis of in vivo complement activation. Herzenberg LA, Weir DM, Herzenberg LA, Blackwell C: Weir’s Handbook of Experimental Immunology. Boston, MA: Blackwell Science, pp. 78.1-78.8.
5. Jansen J, Høgåsen K, Mollnes T (1993) Extensive Complement Activation in Hereditary Porcine Membranoproliferative Glomerulonephritis Type 11 (Porcine Dense Deposit Disease. Am J Path 194: 1356-1365.
ELISA: DIA 011-01 is well suited for quantifying TCC in ELISA. (2,3)
WB: DIA 011-01 is not recommended for Western blotting as the epitope is destroyed during the process. (4)
IHC: TCC in various tissues by immunohistochemistry (frozen and paraffin sections). (1, 2, 4)
IF: DIA 011-01 can be used in immunofluorescence staining. (5)
1. Mollnes TE, Harboe M (1987) Immunohistochemical detection of the membrane and fluid-phase terminal complement complexes C5b-9(m) and SC5b-9. Consequences for interpretation and terminology. Scand J Immunol 26:381-6.
2. Mollnes TE, Lea T, Harboe M, Tschopp J (1985) Monoclonal antibodies recognizing a neoantigen of poly (C9) detect the human terminal complement complex in tissue and plasma. Scand J Immunol 22:183-195.
3. Palarasah,Y, Nielsen C, Sprogøe U, Christensen ML, Lillevang S, Madsen HO, Bygum A, Koch C, Skjodt K, Skjoed MO (2011) Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system. Clin Exp Immun 164: 388-395.
4. Mollnes TE (1997) Analysis of in vivo complement activation. Herzenberg LA, Weir DM, Herzenberg LA, Blackwell C: Weir’s Handbook of Experimental Immunology. Boston, MA: Blackwell Science, pp. 78.1-78.8.
5. Jansen J, Høgåsen K, Mollnes T (1993) Extensive Complement Activation in Hereditary Porcine Membranoproliferative Glomerulonephritis Type 11 (Porcine Dense Deposit Disease. Am J Path 194: 1356-1365.
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Anti-Complement component C5b-9 (human)
CAT. NO. DIA 011-01B