Mouse monoclonal antibody

Anti-Complement factor D (human)

CAT NO: GAU 010-04 | Application: ELISA, WB

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Product Summary

Cat. No.

GAU 010-04

Specificity

GAU 010-04 is specific for complement factor D in human serum and plasma. (1)

Immunogen

Human factor D (1)

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Isotype

IgG2a/k

Gene ID

1675

Clone Number

D10/4

Epitope Specificity

Epitope specificity differs from that of GAU 008-01. (1)

Unit Size

400 µL: Cat. No. GAU 010-04-04 1 mL: Cat. No. GAU 010-04-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Complement factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment.

Application

ELISA: GAU 010-04 can be used as a capture antibody in sandwich ELISA with biotinylated GAU 008-01 as a detection antibody. (1)

WB: GAU 010-04 can be used for Western blotting following SDS-PAGE under non-reducing conditions. (2)

Immunoblot with GAU 010-04: Lane 1: Purified Factor D Lane 2: Factor D in human serum Lane 3: SDS-PAGE purified Factor D Lane 4: MW-marker

Scientific References

1. Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190.

2. Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int. 40:939-947.