Mouse monoclonal antibody, biotinylated

Anti-Complement factor D (human)

CAT NO: GAU 008-01B | Application: ELISA

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Product Summary

Cat. No.

GAU 008-01B

Specificity

GAU 008-01 is specific for complement factor D in human serum and plasma. (1)

Immunogen

Human factor D (1)

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

1675

Clone Number

18/1b

Epitope Specificity

Epitope specificity differs from that of GAU 010-04. (1)

Unit Size

150 µL, 1 mg/mL +/- 15%. Cat. No. GAU 008-01B-015 See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide

Conjugation

Biotin

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Complement factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment.

Application

ELISA: GAU 008-01 is used as a biotinylated detection antibody in sandwich ELISA with a GAU 010-04 capture. (1)

Scientific References

1. Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190.