Mouse monoclonal antibody

Anti-Exendin-4

CAT NO: ABS 012-35 | Application: ELISA

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Product Summary

Cat. No.

ABS 012-35

Specificity

Specific for exendin-4. No cross-reactivity with GLP-1, GLP-2 (human) or glucagon coated on ELISA wells.

Immunogen

Synthetic exendin-4

Host

Mouse

Positive Species Reactivity

Gila monster Heloderma suspectum

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

2740

Clone Number

Epitope Specificity

The epitope is in the 9-39 region of the peptide, and the antibody cross-reacts strongly with exendin (9-39) amide.

Unit Size

400 µL: Cat. No. ABS 012-35-04 1 mL: Cat. No. ABS 012-35-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Application

ELISA: ABS 012-35 binds to exendin-4 when coated on ELISA wells and reacts specifically with exendin-4 in solution giving a Ka of 1.1x10E9 in an inhibition ELISA. The binding between ABS 012-35 and exendin-4 is disrupted by 4.5 M MgCl2.

ABS 012-35 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 033-10 (as capture antibody) for measuring exendin-4, giving a detection limit of as little as 40 pmol/L in an unoptimized buffer assay. (1)