Mouse monoclonal antibody

Anti-Exendin-4

CAT NO: ABS 012-35 | Application: ELISA

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Product Summary

Cat. No.
ABS 012-35
Specificity
Specific for exendin-4. No cross-reactivity with GLP-1, GLP-2 (human) or glucagon coated on ELISA wells.
Immunogen
Synthetic exendin-4
Host
Mouse
Positive Species Reactivity
Gila monster Heloderma suspectum
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
2740
Clone Number
35
Epitope Specificity
The epitope is in the 9-39 region of the peptide, and the antibody cross-reacts strongly with exendin (9-39) amide.
Unit Size
400 µL: Cat. No. ABS 012-35-04 1 mL: Cat. No. ABS 012-35-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.

Application

ELISA: ABS 012-35 binds to exendin-4 when coated on ELISA wells and reacts specifically with exendin-4 in solution giving a Ka of 1.1x10E9 in an inhibition ELISA. The binding between ABS 012-35 and exendin-4 is disrupted by 4.5 M MgCl2.

ABS 012-35 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 033-10 (as capture antibody) for measuring exendin-4, giving a detection limit of as little as 40 pmol/L in an unoptimized buffer assay. (1)

Cat. No.
ABS 012-35
Specificity
Specific for exendin-4. No cross-reactivity with GLP-1, GLP-2 (human) or glucagon coated on ELISA wells.
Immunogen
Synthetic exendin-4
Host
Mouse
Positive Species Reactivity
Gila monster Heloderma suspectum
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
2740
Clone Number
35
Epitope Specificity
The epitope is in the 9-39 region of the peptide, and the antibody cross-reacts strongly with exendin (9-39) amide.
Unit Size
400 µL: Cat. No. ABS 012-35-04 1 mL: Cat. No. ABS 012-35-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.

ELISA: ABS 012-35 binds to exendin-4 when coated on ELISA wells and reacts specifically with exendin-4 in solution giving a Ka of 1.1x10E9 in an inhibition ELISA. The binding between ABS 012-35 and exendin-4 is disrupted by 4.5 M MgCl2.

ABS 012-35 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 033-10 (as capture antibody) for measuring exendin-4, giving a detection limit of as little as 40 pmol/L in an unoptimized buffer assay. (1)

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Product Specification

Non-hazardous statement (SDS)

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