Mouse monoclonal antibody

Anti-GLP-1(7-37) and GLP-1(7-36) amide (N-terminal specific)

CAT NO: ABS 033-04 | Application: ELISA, IHC

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Product Summary

Cat. No.
ABS 033-04
Specificity
ABS 033-04 binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows <0.2% cross-reactivity with GLP-1(1-37), GLP-1(9-36)amide, glucagon, human GIP and exendin-4. Cross-reacts approximately 1% with human GLP-2.
Immunogen
Synthetic GLP-1(7-17)
Host
Mouse
Positive Species Reactivity
Mammalia
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
2641
Clone Number
4
Epitope Specificity
Free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide.
Unit Size
400 µL: Cat. No. ABS 033-04-04 1 mL: Cat. No. ABS 033-04-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Application

ELISA: ABS 033-04 binds free GLP-1(7-37) and free GLP-1(7-36) amide. Not recommended for use in sandwich ELISA. (1)

IHC: ABS 033-04 can be used in IHC.

Endocrine alpha cells from diabetic mice stained with ABS 033-04 (1:500). Courtesy of Dr. Annette Plesner, University of British Columbia.

Scientific References

1. Murphy RE, Kinhikar AG, Shields MJ, Rosario JD, Preston R, Levin N, Ward GH (2010) Combined use of immunoassay and two-dimensional liquid chromatography mass spectrometry for the detection and identification of metabolites from biotherapeutic pharmacokinetic samples. Journal of Pharmaceutical and Biomedical Analysis 53:221-227.

Cat. No.
ABS 033-04
Specificity
ABS 033-04 binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows <0.2% cross-reactivity with GLP-1(1-37), GLP-1(9-36)amide, glucagon, human GIP and exendin-4. Cross-reacts approximately 1% with human GLP-2.
Immunogen
Synthetic GLP-1(7-17)
Host
Mouse
Positive Species Reactivity
Mammalia
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
2641
Clone Number
4
Epitope Specificity
Free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide.
Unit Size
400 µL: Cat. No. ABS 033-04-04 1 mL: Cat. No. ABS 033-04-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

ELISA: ABS 033-04 binds free GLP-1(7-37) and free GLP-1(7-36) amide. Not recommended for use in sandwich ELISA. (1)

IHC: ABS 033-04 can be used in IHC.

Endocrine alpha cells from diabetic mice stained with ABS 033-04 (1:500). Courtesy of Dr. Annette Plesner, University of British Columbia.

1. Murphy RE, Kinhikar AG, Shields MJ, Rosario JD, Preston R, Levin N, Ward GH (2010) Combined use of immunoassay and two-dimensional liquid chromatography mass spectrometry for the detection and identification of metabolites from biotherapeutic pharmacokinetic samples. Journal of Pharmaceutical and Biomedical Analysis 53:221-227.

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Product Specification

Non-hazardous statement (SDS)

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