Mouse monoclonal antibody

Anti-GLP-1(7-37) and GLP-1(7-36) amide (N-terminal specific)

CAT NO: ABS 033-04 | Application: ELISA, IHC

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Product Summary

Cat. No.

ABS 033-04

Specificity

ABS 033-04 binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows <0.2% cross-reactivity with GLP-1(1-37), GLP-1(9-36)amide, glucagon, human GIP and exendin-4. Cross-reacts approximately 1% with human GLP-2.

Immunogen

Synthetic GLP-1(7-17)

Host

Mouse

Positive Species Reactivity

Mammalia

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

2641

Clone Number

Epitope Specificity

Free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide.

Unit Size

400 µL: Cat. No. ABS 033-04-04 1 mL: Cat. No. ABS 033-04-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Application

ELISA: ABS 033-04 binds free GLP-1(7-37) and free GLP-1(7-36) amide. Not recommended for use in sandwich ELISA. (1)

IHC: ABS 033-04 can be used in IHC.

Endocrine alpha cells from diabetic mice stained with ABS 033-04 (1:500). Courtesy of Dr. Annette Plesner, University of British Columbia.

Scientific References

1. Murphy RE, Kinhikar AG, Shields MJ, Rosario JD, Preston R, Levin N, Ward GH (2010) Combined use of immunoassay and two-dimensional liquid chromatography mass spectrometry for the detection and identification of metabolites from biotherapeutic pharmacokinetic samples. Journal of Pharmaceutical and Biomedical Analysis 53:221-227.