Mouse monoclonal antibody
Anti-GLP-1(7-37) and GLP-1(7-36) amide (N-terminal specific)
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Product Summary
Application
ELISA: ABS 033-10 binds free GLP-1(7-37) and free GLP-1(7-36)amide in solution. ABS 033-10 reacts in ELISA with GLP-1(7-37) coated directly onto the microtiter well. ABS 033-10 binds free exendin-4 in solution and can function as a capture antibody for exendin-4 in combination with ABS 012-35B in order to measure Exendin-4. When measuring “active” GLP-1 ABS 033-10B works as a detection antibody in combination with HYB 147-12. ABS 033-10B can be used as a detection antibody in combination with ABS 046-03 as a capture antibody in order to measure non-amidated GLP-1 forms and cross-reacting about 5% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.
WB: ABS 033-10 can be used in Western blotting.
ELISA: ABS 033-10 binds free GLP-1(7-37) and free GLP-1(7-36)amide in solution. ABS 033-10 reacts in ELISA with GLP-1(7-37) coated directly onto the microtiter well. ABS 033-10 binds free exendin-4 in solution and can function as a capture antibody for exendin-4 in combination with ABS 012-35B in order to measure Exendin-4. When measuring “active” GLP-1 ABS 033-10B works as a detection antibody in combination with HYB 147-12. ABS 033-10B can be used as a detection antibody in combination with ABS 046-03 as a capture antibody in order to measure non-amidated GLP-1 forms and cross-reacting about 5% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.
WB: ABS 033-10 can be used in Western blotting.
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