Mouse monoclonal antibody

Anti-GLP-1(7-37) and GLP-1(7-36) amide (N-terminal specific)

CAT NO: ABS 033-10 | Application: ELISA, WB

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Product Summary

Cat. No.

ABS 033-10

Specificity

ABS 033-10 binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows <0.2% cross-reactivity with GLP-1(9-36)amide, glucagon and human GIP. Cross-reacts 0.25% with GLP-1(1-37), approximately 6% with human GLP-2 and approximately 50% with exendin-4. ABS 033-10 cross-reacts with Liraglutide.

Immunogen

Synthetic GLP-1(7-17)

Host

Mouse

Positive Species Reactivity

Mammalia

Negative Species Reactivity

Not determined

Isotype

IgG2a/k

Gene ID

2641

Clone Number

Epitope Specificity

Free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide.

Unit Size

400 µL: Cat. No. ABS 033-10-04 1 mL: Cat. No. ABS 033-10-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Application

ELISA: ABS 033-10 binds free GLP-1(7-37) and free GLP-1(7-36)amide in solution. ABS 033-10 reacts in ELISA with GLP-1(7-37) coated directly onto the microtiter well.

ABS 033-10 binds free exendin-4 in solution and can function as a capture antibody for exendin-4 in combination with ABS 012-35B in order to measure Exendin-4.

When measuring “active” GLP-1 ABS 033-10B works as a detection antibody in combination with HYB 147-12. ABS 033-10B can be used as a detection antibody in combination with ABS 046-03 as a capture antibody in order to measure non-amidated GLP-1 forms and cross-reacting about 5% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.

WB: ABS 033-10 can be used in Western blotting.