Mouse monoclonal antibody

Anti-Fibronectin (bovine, human, chicken)

CAT NO: CSI 005-17 | Application: ELISA, WB, IHC-F, IHC-P, IP

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Product Summary

Cat. No.

CSI 005-17

Specificity

CSI 005-17 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). The antibody inhibits integrin-mediated cell adhesion to the cell binding domain of fibronectin.

Immunogen

Lysed bovine corneal endothelial cells and extracellular matrix

Host

Mouse

Positive Species Reactivity

Bovine, Human, Chicken

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

280794 (2335, 396133)

Clone Number

A17

Epitope Specificity

Epitope is located in the 120kD cell binding fragment

Unit Size

400 µL: Cat. No. CSI 005-17-04 1 mL: Cat. No. CSI 005-17-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities. (1)

Application

ELISA: CSI 005-17 can be used in ELISA. (1, 2, 3, 4, 5)

WB: In Western blotting a dilution guideline of 1/100 has proved successful. (1)

IHC-F: CSI 005-17 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.

IHC-P: CSI 005-17 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.

IP: CSI 005-17 can be used in immunoprecipitation. It can be used to probe fibronectin conformation.

Scientific References

1. Underwood PA, Dalton BA, Steele JG, Bennett FA, Strike P (1992) Anti-fibronectin antibodies that modify heparin binding and cell adhesion: evidence for a new cell binding site in the heparin binding region. J Cell Sci 102:833-845.

2. Underwood PA, Steele JG, Dalton BA (1993) Effects of polystyrene surface chemistry on biological activity of solid phase fibronectin and vitronectin, analysed with monoclonal antibodies. J Cell Sci 104:793-803.

3. Di Girolamo N, Underwood PA, McCluskey PJ, Wakefield D (1993) Functional activity of plasma fibronectin in patients with Diabetes mellitis. Diabetes 42:1606-1613.

4. Dalton BA, McFarland CD, Underwood PA, Steele JG (1995) Role of heparin binding domain of fibronectin in attachment and spreading of human bone derived cells. J Cell Sci 108:2083-2092.

5. Underwood PA, Bean PA, Mitchell SM, Whitelock JM (2001) Specific affinity depletion of cell adhesion molecules and growth factors from serum. J Immunol Methods 247:217-224.