Mouse monoclonal antibody

Anti-Fibronectin (bovine, human)

CAT NO: CSI 005-32 | Application: ELISA, WB, IHC-F, IHC-P, IP

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Product Summary

Cat. No.

CSI 005-32

Specificity

CSI 005-32 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). The antibody inhibits cell adhesion to fibronectin mediated by the heparin-binding domain.

Immunogen

Bovine corneal endothelial cells

Host

Mouse

Positive Species Reactivity

Bovine, Human

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

280794 (2335)

Clone Number

A32

Epitope Specificity

Epitope is located in the 40kD Hep II heparin-binding domain, but differs from that of CSI 005-35

Unit Size

400 µL: Cat. No. CSI 005-32-04 1 mL: Cat. No. CSI 005-32-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities. (1)

Application

ELISA: CSI 005-32 can be used in ELISA. It can be used to probe fibronectin conformation and to quantitate plasma fibronectin in a sandwich ELISA with antibody CSI 005-35. (1, 2, 3, 4)

WB: In Western blotting dilution guideline of 1/100 has proved successful. (1)

IHC-F: CSI 005-32 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.

IHC-P: CSI 005-32 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.

IP: CSI 005-32 can be used in immunoprecipitation.

Scientific References

1. Underwood PA, Dalton BA, Steele JG, Bennett FA, Strike P (1992) Anti-fibronectin antibodies that modify heparin binding and cell adhesion: evidence for a new cell binding site in the heparin binding region. J Cell Sci 102:833-845.

2. Underwood PA, Steele JG, Dalton BA (1993) Effects of polystyrene surface chemistry on biological activity of solid phase fibronectin and vitronectin, analysed with monoclonal antibodies. J Cell Sci 104:793-803.

3. Di Girolamo N, Underwood PA, McCluskey PJ, Wakefield D (1993) Functional activity of plasma fibronectin in patients with Diabetes mellitis. Diabetes 42:1606-1613.

4. Dalton BA, McFarland CD, Underwood PA, Steele JG (1995) Role of heparin binding domain of fibronectin in attachment and spreading of human bone derived cells. J Cell Sci 108:2083-2092.