Mouse monoclonal antibody
Anti-Type V Collagen (human, dog, sheep, kangaroo, pig, rabbit, bovine)
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Product Summary
Application
ELISA: CSI 006-01 can be used for detection of collagens by ELISA. CSI 006-01 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
WB: In immunoblotting CSI 006-01 detects human type V collagen only in its native triple helix form.
IHC-P: CSI 006-01 has been used succesfully for immunohistology on paraffin embedded (1) and frozen unfixed sections of human (2), bovine (3) and dog (4) skin, on rabbit (1) and foetal bovine cornea (4), and of new dog tissue associated with a biomaterial implant (5). If fixation of tissue is required, acetone or ethanol is recommended.
IHC-F: CSI 006-01 has been used succesfully for immunohistology on paraffin embedded (1) and frozen unfixed sections of human (2), bovine (3) and dog (4) skin, on rabbit (1) and foetal bovine cornea (4), and of new dog tissue associated with a biomaterial implant (5). If fixation of tissue is required, acetone or ethanol is recommended.
Scientific References
1. Pollock GA, McKelvie PA, McCarty DJ, White JF, Mallari PL, Taylor HR. (2003) In vivo effects of fluoroquinolones on rabbit corneas. Clin Experiment Ophthalmol 31:517-21.
2. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402.
3. Werkmeister JA, Ramshaw JAM (1988) The use of immunohistology in studies on connective tissue organisation in hides and skins. Das Leder 39:145-151.
4. Werkmeister JA, Ramshaw JAM (1991) Monoclonal antibodies to type V collagen as markers for new tissue deposition associated with biomaterial implants. J Histochem Cytochem 39:1215-1220.
5. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.
ELISA: CSI 006-01 can be used for detection of collagens by ELISA. CSI 006-01 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
WB: In immunoblotting CSI 006-01 detects human type V collagen only in its native triple helix form.
IHC-P: CSI 006-01 has been used succesfully for immunohistology on paraffin embedded (1) and frozen unfixed sections of human (2), bovine (3) and dog (4) skin, on rabbit (1) and foetal bovine cornea (4), and of new dog tissue associated with a biomaterial implant (5). If fixation of tissue is required, acetone or ethanol is recommended.
IHC-F: CSI 006-01 has been used succesfully for immunohistology on paraffin embedded (1) and frozen unfixed sections of human (2), bovine (3) and dog (4) skin, on rabbit (1) and foetal bovine cornea (4), and of new dog tissue associated with a biomaterial implant (5). If fixation of tissue is required, acetone or ethanol is recommended.
1. Pollock GA, McKelvie PA, McCarty DJ, White JF, Mallari PL, Taylor HR. (2003) In vivo effects of fluoroquinolones on rabbit corneas. Clin Experiment Ophthalmol 31:517-21.
2. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402.
3. Werkmeister JA, Ramshaw JAM (1988) The use of immunohistology in studies on connective tissue organisation in hides and skins. Das Leder 39:145-151.
4. Werkmeister JA, Ramshaw JAM (1991) Monoclonal antibodies to type V collagen as markers for new tissue deposition associated with biomaterial implants. J Histochem Cytochem 39:1215-1220.
5. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.
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