Mouse monoclonal antibody

Anti-Type V Collagen (human, dog, sheep, kangaroo, pig, rabbit, bovine)

CAT NO: CSI 006-01 | Application: ELISA, WB, IHC-P, IHC-F

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Product Summary

Cat. No.

CSI 006-01

Specificity

CSI 006-01 is highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).

Immunogen

Acid-digested pepsin soluble dog type V collagen

Host

Mouse

Positive Species Reactivity

Human, Pig, Bovine, Dog, Sheep (ovine), Kangaroo, Rabbit

Negative Species Reactivity

Mouse, Rat, Guinea Pig, Chicken

Isotype

IgG2a/k

Gene ID

1289,397533, 537387, 480684, 101115457, 100327268

Clone Number

1E2-E4/Col5

Epitope Specificity

Not determined

Unit Size

400 µL: Cat. No. CSI 006-01-04 1 mL: Cat. No. CSI 006-01-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Type V collagen is a minor component of the connective tissue, although it is present in many different types of connective tissue. Patients with defects in the type V collagen (Ehlers-Danlos syndrome) have weakend connective tissue characterized by hyperstrechable joints and fragile, easily bruisable skin.

Application

ELISA: CSI 006-01 can be used for detection of collagens by ELISA. CSI 006-01 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.

WB: In immunoblotting CSI 006-01 detects human type V collagen only in its native triple helix form.

IHC-P: CSI 006-01 has been used succesfully for immunohistology on paraffin embedded (1) and frozen unfixed sections of human (2), bovine (3) and dog (4) skin, on rabbit (1) and foetal bovine cornea (4), and of new dog tissue associated with a biomaterial implant (5). If fixation of tissue is required, acetone or ethanol is recommended.

IHC-F: CSI 006-01 has been used succesfully for immunohistology on paraffin embedded (1) and frozen unfixed sections of human (2), bovine (3) and dog (4) skin, on rabbit (1) and foetal bovine cornea (4), and of new dog tissue associated with a biomaterial implant (5). If fixation of tissue is required, acetone or ethanol is recommended.

Scientific References

1. Pollock GA, McKelvie PA, McCarty DJ, White JF, Mallari PL, Taylor HR. (2003) In vivo effects of fluoroquinolones on rabbit corneas. Clin Experiment Ophthalmol 31:517-21.

2. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402.

3. Werkmeister JA, Ramshaw JAM (1988) The use of immunohistology in studies on connective tissue organisation in hides and skins. Das Leder 39:145-151.

4. Werkmeister JA, Ramshaw JAM (1991) Monoclonal antibodies to type V collagen as markers for new tissue deposition associated with biomaterial implants. J Histochem Cytochem 39:1215-1220.

5. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.