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Anti-Complement component C9 (human)

CAT NO: ABS 004-22

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Product Name	Anti-Complement component C9 (human)
Description	Mouse monoclonal antibody
Isotype	IgG1/k
Host	Mouse
Clone	22
Conjugation	Unconjugated
Tested Applications	ELISA, WB, IHC, IF

ELISA	ABS 004-22 recognizes C9 in human serum diluted 1/50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37°C using a human IgM coated (10 μg/mL overnight at 4°C, blocked with PBS 7.2 + 1% BSA for 1 hour) ELISA plate.
WB	ABS 004-22 was used in Western blotting.
IHC	ABS 004-22 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:100.
	IHC staining of human liver with ABS 004-22. Plasma of portal veins are strongly stained. Dilution: 1:100.	IHC staining of tonsil tissue with ABS 004-22. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue. Also moderate staining were observed from plasma of veins. Dilution: 1:100.
IF	ABS 004-22 was used in IF with a dilution of 1:75.
	IF staining of tonsil tissue with ABS 004-22. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue. Dilution: 1:75.	IF staining of human kidney with ABS 004-22. Plasma of veins are strongly stained. Dilution: 1:75.