Anti-yENaC (y−subunit epithelial sodium channel)

CAT NO: CAM 003-07

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Product Name	Anti-yENaC (y−subunit epithelial sodium channel)
Description	Mouse monoclonal antibody
Isotype	IgG1/k
Host	Mouse
Clone	3c7
Conjugation	Unconjugated
Tested Applications	ELISA, WB, IHC

ELISA	CAM 003-07 was used in ELISA. A sandwich ELISA can be made using CAM 003-07 (1-4 μg/ml) as the capture antibody and biotinylated CAM 005-02 (0.05 - 0.2 μg/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131).
WB	CAM 003-07 was used in Western blot. (1,2)
	WB performed with CAM 003-07 on a lysate of HEK293 cells transfected with and without γENaC. Lane 1 loaded with 5µg, Lane 2 loaded with 10µg, Lane 3 loaded with 20 µg.
IHC	CAM 003-07 was used in immunohistochemistry.

Specificity
Specificity	CAM 003-07 is specific for the inhibitory tract of human yENaC subunit.The epitope differs from that of CAM 005-02
Immunogen	The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human yENaC subunit.
Target	The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the y-subunit and putative release of a 43-amino acid inhibitory tract from the y-subunit ectodomain.
Gene ID	6340
Species Reactivity POSITIVE	Human
Species Reactivity NEGATIVE	Not determined

Properties
Form	Liquid
Unit Size	0,4 mL and 1 mL
Concentration	1 mg/mL ±15%, See CoA for lot details
Purification	Protein A or Protein G purified
Purification Notes	BSA free
Storage buffer	0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide