APC-PCI Complex specific antibody

CAT NO: JST 001-38

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Product Name	APC-PCI Complex specific antibody
Description	Mouse monoclonal antibody
Isotype	IgG1/k
Host	Mouse
Clone	M36
Conjugation	Unconjugated
Tested Applications	ELISA, WB

Specificity
Specificity	JST 001-38 is specific for APC:PCI complex and cleaved PCI but has little reactivity towards native PCI. The dissociation constants (as determined by standard surface plasmon resonance technique) are: Kd= 4x10e-10 M for PCI in complex with APC and 2x10e-10 M for cleaved PCI. The value of Kd for native PCI is too low to measure, i.e. > 10e-5 M.The epitope is loctated in the calcium-binding N-terminal EGF domain of protein C.
Immunogen	Mixture of PCI in complex with PSA (approx. 80%) and PCI cleaved from such a complex (approx. 20%).
Target	Activated Protein C (APC), a serine proteinase is inhibited by Protein C inhibitor (PCI). PCI belongs to a group of inhibitors sometimes referred to as serpines (serine proteinase inhibitors), and form 1:1 complexes with APC. PCI plasma concentration is approximately 4ug/m1 .The complex formation between APC and PCI proceeds at a slow rate, which is manifested by a long half-life for APC in plasma, wherein t1/2 is about 20 min.However, the rate of complex formation between APC and PCI is increased by heparin.Upon complex formation with APC, the serpin is cleaved in its so-called bait region, whereby a stable intermediate acyl complex is formed. With time, the intermediate acyl complex dissociates, whereby APC is regenerated and a proteolytically modified, i. e. cleaved, inactive serine proteinase inhibitor is formed.
Gene ID	5104
Species Reactivity POSITIVE	Human
Species Reactivity NEGATIVE	Not determined

Properties
Form	Liquid
Unit Size	0,4 mL and 1 mL
Concentration	1 mg/mL ±15%, See CoA for lot details
Purification	Protein A or Protein G purified
Purification Notes	BSA free
Storage buffer	0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

ELISA	JST 001-38 shows excellent reactivity towards PCI in cleaved and complex form in ELISA. (1-5)
WB	JST 001-38 shows low reactivity against denatured antigen in SDS-PAGE.