Mouse monoclonal antibody

Anti-Complement component C9 (human)

CAT NO: ABS 004-22 | Application: ELISA, WB, IHC, IF

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Product Summary

Cat. No.
ABS 004-22
Specificity
ABS 004-22 is specific for complement factor C9.
Immunogen
Complement factor C9
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
735
Clone Number
22
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. ABS 004-22-04 1 mL: Cat. No. ABS 004-22-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A/G purified, from culture supernatant
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Complement factor C9 is a 71kDa single-chain ellipsoid a-globular molecule of 558 amino acids including a leader sequence of 20 amino acids. C9 bind the C5b8 complex on membranes and has an unique property in forming tubular structures containing between 12 and 18 monomers, closely resembling the membrane attack complex (MAC). The primary site of synthesis is hepatocytes, secondary sites are monocytes, fibroblasts and glial cells. The normal level of C9 in plasma is 60 µg/mL, and the level is up regulated from hepatocytes by IFN-g.

Application

ELISA: ABS 004-22 recognizes C9 in human serum diluted 1/50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37°C using a human IgM coated (10 μg/mL overnight at 4°C, blocked with PBS 7.2 + 1% BSA for 1 hour) ELISA plate.

WB: ABS 004-22 was used in Western blotting.

IHC: ABS 004-22 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:100.

IHC staining of human liver with ABS 004-22. Plasma of portal veins are strongly stained. Dilution: 1:100.

IHC staining of tonsil tissue with ABS 004-22. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue. Also moderate staining were observed from plasma of veins. Dilution: 1:100.

IF: ABS 004-22 was used in IF with a dilution of 1:75.

IF staining of tonsil tissue with ABS 004-22. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue. Dilution: 1:75.

IF staining of human kidney with ABS 004-22. Plasma of veins are strongly stained. Dilution: 1:75.

Cat. No.
ABS 004-22
Specificity
ABS 004-22 is specific for complement factor C9.
Immunogen
Complement factor C9
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
735
Clone Number
22
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. ABS 004-22-04 1 mL: Cat. No. ABS 004-22-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A/G purified, from culture supernatant
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Complement factor C9 is a 71kDa single-chain ellipsoid a-globular molecule of 558 amino acids including a leader sequence of 20 amino acids. C9 bind the C5b8 complex on membranes and has an unique property in forming tubular structures containing between 12 and 18 monomers, closely resembling the membrane attack complex (MAC). The primary site of synthesis is hepatocytes, secondary sites are monocytes, fibroblasts and glial cells. The normal level of C9 in plasma is 60 µg/mL, and the level is up regulated from hepatocytes by IFN-g.

ELISA: ABS 004-22 recognizes C9 in human serum diluted 1/50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37°C using a human IgM coated (10 μg/mL overnight at 4°C, blocked with PBS 7.2 + 1% BSA for 1 hour) ELISA plate.

WB: ABS 004-22 was used in Western blotting.

IHC: ABS 004-22 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:100.

IHC staining of human liver with ABS 004-22. Plasma of portal veins are strongly stained. Dilution: 1:100.

IHC staining of tonsil tissue with ABS 004-22. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue. Also moderate staining were observed from plasma of veins. Dilution: 1:100.

IF: ABS 004-22 was used in IF with a dilution of 1:75.

IF staining of tonsil tissue with ABS 004-22. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue. Dilution: 1:75.

IF staining of human kidney with ABS 004-22. Plasma of veins are strongly stained. Dilution: 1:75.

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Product Specification

Non-hazardous statement (SDS)

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