Mouse monoclonal antibody

Anti-GLP-1 (Non-amidated (-Arg-Gly), C-terminal specific)

CAT NO: ABS 046-03 | Application: ELISA, WB

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Product Summary

Cat. No.
ABS 046-03
Specificity
ABS 046-03 binds GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37). Cross-reaction with C-terminally amidated GLP-1 is 0-16% in various circumstances.
Immunogen
Synthetic GLP-1(9-37)
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
2641
Clone Number
03
Epitope Specificity
C-terminus of GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37)
Unit Size
400 µL: Cat. No. ABS 046-03-04 1 mL: Cat. No. ABS 046-03-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
GLP-1(7-36) amide is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Application

ELISA: ABS 046-03 binds free GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37) in solution. ABS 046-03 binds free GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37) in solution. ABS 046-03 works well as a capture antibody with ABS 033-10B as a biotinylated detection antibody cross-reacting about 5% with C-terminally amidated GLP-1. ABS 046-03 can also be used as a capture antibody for detection of GLP-1 derivatives. (1)

WB: ABS 046-03 can be used in Western blotting.

Scientific References

1. Sang Y, Zhou T, Li H, Ran Y, Jiang D, Zheng F, Chen Y, Wang C, Zou X (2013) A new long-acting GLP-1 derivative KTP ameliorates hyperglycemia and dyslipidemia and improves pancreas and fatty liver in db/db mice. Chinese Science Bulletin 58:2447-2453

Cat. No.
ABS 046-03
Specificity
ABS 046-03 binds GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37). Cross-reaction with C-terminally amidated GLP-1 is 0-16% in various circumstances.
Immunogen
Synthetic GLP-1(9-37)
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
2641
Clone Number
03
Epitope Specificity
C-terminus of GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37)
Unit Size
400 µL: Cat. No. ABS 046-03-04 1 mL: Cat. No. ABS 046-03-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
GLP-1(7-36) amide is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

ELISA: ABS 046-03 binds free GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37) in solution. ABS 046-03 binds free GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37) in solution. ABS 046-03 works well as a capture antibody with ABS 033-10B as a biotinylated detection antibody cross-reacting about 5% with C-terminally amidated GLP-1. ABS 046-03 can also be used as a capture antibody for detection of GLP-1 derivatives. (1)

WB: ABS 046-03 can be used in Western blotting.

1. Sang Y, Zhou T, Li H, Ran Y, Jiang D, Zheng F, Chen Y, Wang C, Zou X (2013) A new long-acting GLP-1 derivative KTP ameliorates hyperglycemia and dyslipidemia and improves pancreas and fatty liver in db/db mice. Chinese Science Bulletin 58:2447-2453

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Product Specification

Non-hazardous statement (SDS)

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