Mouse monoclonal antibody, biotinylated

Anti-GLP-1 (Non-amidated (-Arg-Gly), C-terminal specific)

CAT NO: ABS 046-03B | Application: ELISA

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Product Summary

Cat. No.

ABS 046-03B

Specificity

ABS 046-03 binds GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37). Cross-reaction with C-terminally amidated GLP-1 is 0-16% in various circumstances.

Immunogen

Synthetic GLP-1(9-37)

Host

Mouse

Positive Species Reactivity

Mammalia

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

2641

Clone Number

Epitope Specificity

C-terminus of GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37)

Unit Size

150 µL: Cat. No. ABS 046-03B-015 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Biotinylated

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide

Conjugation

Biotin

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

GLP-1(7-36) amide is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Application

ELISA: ABS 046-03B as a biotinylated detection antibody and HYB 147-12 as a capture antibody cross-reacting about 16% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.