Mouse monoclonal antibody, biotinylated

Anti-GLP-1 (Non-amidated (-Arg-Gly), C-terminal specific)

CAT NO: ABS 046-03B | Application: ELISA

Request Price
×

Request Price

"*" indicates required fields

Name*
Consent*
This field is for validation purposes and should be left unchanged.

Product Summary

Cat. No.
ABS 046-03B
Specificity
ABS 046-03 binds GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37). Cross-reaction with C-terminally amidated GLP-1 is 0-16% in various circumstances.
Immunogen
Synthetic GLP-1(9-37)
Host
Mouse
Positive Species Reactivity
Mammalia
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
2641
Clone Number
3
Epitope Specificity
C-terminus of GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37)
Unit Size
150 µL: Cat. No. ABS 046-03B-015 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide
Conjugation
Biotin
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
GLP-1(7-36) amide is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Application

ELISA: ABS 046-03B as a biotinylated detection antibody and HYB 147-12 as a capture antibody cross-reacting about 16% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.

Cat. No.
ABS 046-03B
Specificity
ABS 046-03 binds GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37). Cross-reaction with C-terminally amidated GLP-1 is 0-16% in various circumstances.
Immunogen
Synthetic GLP-1(9-37)
Host
Mouse
Positive Species Reactivity
Mammalia
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
2641
Clone Number
3
Epitope Specificity
C-terminus of GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37)
Unit Size
150 µL: Cat. No. ABS 046-03B-015 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15mM sodium azide
Conjugation
Biotin
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
GLP-1(7-36) amide is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

ELISA: ABS 046-03B as a biotinylated detection antibody and HYB 147-12 as a capture antibody cross-reacting about 16% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.

Downloads

+

Product Specification

Non-hazardous statement (SDS)

Related Products

+

>

Anti-GLP-1(7-37) and GLP-1(7-36) amide (N-terminal specific)

CAT. NO. ABS 033-10B

>

Anti-GLP-1(7-37) and GLP-1(7-36) amide (N-terminal specific)

CAT. NO. ABS 033-10

>

Anti-GLP-1 (Non-amidated (-Arg-Gly), C-terminal specific)

CAT. NO. ABS 046-03

×