Mouse monoclonal antibody

Anti-NGAL (dog)

CAT NO: ABS 047-14 | Application: ELISA, WB, IHC

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Product Summary

Cat. No.

ABS 047-14

Specificity

ABS 047-14 binds dog NGAL (neutrophil gelatinase-associated lipocalin).

Immunogen

Recombinant dog NGAL

Host

Mouse

Positive Species Reactivity

Dog

Negative Species Reactivity

Human, Mouse, Rat

Isotype

IgG2b/k

Gene ID

491320

Clone Number

Epitope Specificity

Not determined

Unit Size

400 µL: Cat. No. ABS 047-14-04 1 mL: Cat. No. ABS 047-14-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Dog neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically from renal tubular cells in response to ischemic or nephrotoxic injury.

Application

ELISA: ABS 047-14 binds free dog NGAL. ABS 047-14 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 047-17 (as capture antibody) for measuring dog NGAL, giving a detection limit of 5 pg/mL in an unoptimized buffer assay.

WB: ABS 047-14 can be used in Western blotting.

IHC: ABS 047-14 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:50.

IHC staining in dog kidney tissue. Scattered tubules cells were stained strongly from kidney with acute nephritis. Dilution: 1:50