Mouse monoclonal antibody

Anti-NGAL (dog)

CAT NO: ABS 047-14 | Application: ELISA, WB, IHC

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Product Summary

Cat. No.
ABS 047-14
Specificity
ABS 047-14 binds dog NGAL (neutrophil gelatinase-associated lipocalin).
Immunogen
Recombinant dog NGAL
Host
Mouse
Positive Species Reactivity
Dog
Negative Species Reactivity
Human, Mouse, Rat
Isotype
IgG2b/k
Gene ID
491320
Clone Number
14
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. ABS 047-14-04 1 mL: Cat. No. ABS 047-14-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Dog neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically from renal tubular cells in response to ischemic or nephrotoxic injury.

Application

ELISA: ABS 047-14 binds free dog NGAL. ABS 047-14 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 047-17 (as capture antibody) for measuring dog NGAL, giving a detection limit of 5 pg/mL in an unoptimized buffer assay.

WB: ABS 047-14 can be used in Western blotting.

IHC: ABS 047-14 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:50.

IHC staining in dog kidney tissue. Scattered tubules cells were stained strongly from kidney with acute nephritis. Dilution: 1:50

Cat. No.
ABS 047-14
Specificity
ABS 047-14 binds dog NGAL (neutrophil gelatinase-associated lipocalin).
Immunogen
Recombinant dog NGAL
Host
Mouse
Positive Species Reactivity
Dog
Negative Species Reactivity
Human, Mouse, Rat
Isotype
IgG2b/k
Gene ID
491320
Clone Number
14
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. ABS 047-14-04 1 mL: Cat. No. ABS 047-14-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Dog neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically from renal tubular cells in response to ischemic or nephrotoxic injury.

ELISA: ABS 047-14 binds free dog NGAL. ABS 047-14 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 047-17 (as capture antibody) for measuring dog NGAL, giving a detection limit of 5 pg/mL in an unoptimized buffer assay.

WB: ABS 047-14 can be used in Western blotting.

IHC: ABS 047-14 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:50.

IHC staining in dog kidney tissue. Scattered tubules cells were stained strongly from kidney with acute nephritis. Dilution: 1:50

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Product Specifications

NGAL Product Overview

Non-hazardous statement (SDS)

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