Mouse monoclonal antibody

Anti-NGAL (human)

CAT NO: ABS 038-15 | Application: ELISA, WB

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Product Summary

Cat. No.

ABS 038-15

Specificity

ABS 038-15 binds human NGAL (neutrophil gelatinase-associated lipocalin).

Immunogen

Recombinant human NGAL

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Dog, Pig, Mouse, Rat

Isotype

IgG1/k

Gene ID

3934

Clone Number

Epitope Specificity

Differs from those of HYB 211 and the ABS 062 series of antibodies.

Unit Size

400 µL: Cat. No. ABS 038-15-04 1 mL: Cat. No. ABS 038-15-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by acute kidney injury.

Application

ELISA: ABS 038-15 binds human NGAL in solution and coated onto a solid phase. ABS 038-15 (as capture antibody) forms sandwich pairs with HYB 211-02, ABS 038-04, ABS 038-14 and ABS 038-26 (as biotinylated detection antibodies) for measuring human NGAL. When ABS 038-15 is used as biotinylated detection antibody with ABS 038-14 as capture antibody, the assay reacts equally with NGAL monomer and homodimer on a mass basis.

WB: ABS 038-15 can be used in Western blotting.