Mouse monoclonal antibody

Anti-NGAL (monkey)

CAT NO: ABS 063-56 | Application: ELISA, IHC-P

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Product Summary

Cat. No.
ABS 063-56
Specificity
ABS 063-56 reacts with NGAL (neutrophil gelatinase-associated lipocalin) from Macaca mulatta (Rhesus) and Macaca facicularis (Cynomolgus).
Immunogen
Recombinant monkey NGAL
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
3934
Clone Number
56
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. ABS 063-56-04 1 mL: Cat. No. ABS 063-56-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Monkey neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically from renal tubular cells in response to ischemic or nephrotoxic injury.

Application

ELISA: ABS 063-56 binds free monkey NGAL ABS 063-56 (as capture antibody) forms a sandwich ELISA pair with ABS 063-68 (as biotinylated detection antibody) for measuring monkey NGAL.

IHC-P: ABS 063-56 reacts with monkey NGAL in IHC paraffin sections.

IHC staining for nephrotoxicant-induced NGAL protein expression in male Cynomolgus monkey using the BioPorto Diagnostics antibody (ABS 063-56) at 1:50. Mild multifocal staining was localized in apical surface of intact tubular epithelial cells in the cortex and medulla (left panel) and marked multifocal tubular cast (granular and cellular) in the cortex (right panel). Image: Kindly provided by McDuffie, JE, JY Ma, et al (Janssen Research & Development, LLC)

Scientific References

1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A

Cat. No.
ABS 063-56
Specificity
ABS 063-56 reacts with NGAL (neutrophil gelatinase-associated lipocalin) from Macaca mulatta (Rhesus) and Macaca facicularis (Cynomolgus).
Immunogen
Recombinant monkey NGAL
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
3934
Clone Number
56
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. ABS 063-56-04 1 mL: Cat. No. ABS 063-56-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Monkey neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically from renal tubular cells in response to ischemic or nephrotoxic injury.

ELISA: ABS 063-56 binds free monkey NGAL ABS 063-56 (as capture antibody) forms a sandwich ELISA pair with ABS 063-68 (as biotinylated detection antibody) for measuring monkey NGAL.

IHC-P: ABS 063-56 reacts with monkey NGAL in IHC paraffin sections.

IHC staining for nephrotoxicant-induced NGAL protein expression in male Cynomolgus monkey using the BioPorto Diagnostics antibody (ABS 063-56) at 1:50. Mild multifocal staining was localized in apical surface of intact tubular epithelial cells in the cortex and medulla (left panel) and marked multifocal tubular cast (granular and cellular) in the cortex (right panel). Image: Kindly provided by McDuffie, JE, JY Ma, et al (Janssen Research & Development, LLC)

1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A

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Product Specification

PSTC Summary Report

NGAL Product Overview

Non-hazardous statement (SDS)

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