Rat monoclonal antibody

Anti-NGAL (mouse)

CAT NO: ABS 043-29 | Application: ELISA, WB, IHC-P

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Product Summary

Cat. No.
ABS 043-29
Specificity
ABS 043-29 binds NGAL (neutrophil gelatinase-associated lipocalin) in mouse serum.
Immunogen
Recombinant mouse NGAL
Host
Rat
Positive Species Reactivity
Mouse (murine)
Negative Species Reactivity
Human, Pig, Dog, Rat
Isotype
IgG1/k
Gene ID
16819
Clone Number
29
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. ABS 043-29-04 1 mL: Cat. No. ABS 043-29-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Mouse neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, 24p3, SIP24, uterocalin and siderocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. 24p3 was observed to be dramatically upregulated in kidney cell cultures infected with SV40 and subsequently identified as a lipocalin. Unlike human NGAL, it is an acute phase protein upregulated in the liver, but like its mammalian homologues it is upregulated and secreted by the kidney in response to ischemic or nephrotoxic injury. It is also upregulated in the involuting uterus and mammary gland.

Application

ELISA: ABS 043-29 binds free mouse NGAL in solution. ABS 043-29 forms an antibody pair for sandwich ELISA with ABS 043-10. It is recommended to use ABS 043-10 as capture antibody and biotinylated ABS 043-29 as a detection antibody.

WB: ABS 043-29 can be used in Western blotting.

IHC-P: ABS 043-29 detects NGAL in paraffin-embedded sections in ischemia-reperfusion injured kidney cells from mouse (dilution 1:1000). Microwave pretreatment using Citrat buffer pH 6, is recommended.

NGAL expression in the ischemia-reperfusion injured mouse kidney;detected with anti-mouse NGAL antibody (ABS 043-29; antibody dilution 1:1000). Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.

NGAL expression 2 days after the introduction of ischemic-reperfusion injured mouse kidney;detected with anti-mouse NGAL antibody (ABS 043-29; antibody dilution 1:1000). Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.

Scientific References

1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A

Cat. No.
ABS 043-29
Specificity
ABS 043-29 binds NGAL (neutrophil gelatinase-associated lipocalin) in mouse serum.
Immunogen
Recombinant mouse NGAL
Host
Rat
Positive Species Reactivity
Mouse (murine)
Negative Species Reactivity
Human, Pig, Dog, Rat
Isotype
IgG1/k
Gene ID
16819
Clone Number
29
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. ABS 043-29-04 1 mL: Cat. No. ABS 043-29-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Mouse neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, 24p3, SIP24, uterocalin and siderocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. 24p3 was observed to be dramatically upregulated in kidney cell cultures infected with SV40 and subsequently identified as a lipocalin. Unlike human NGAL, it is an acute phase protein upregulated in the liver, but like its mammalian homologues it is upregulated and secreted by the kidney in response to ischemic or nephrotoxic injury. It is also upregulated in the involuting uterus and mammary gland.

ELISA: ABS 043-29 binds free mouse NGAL in solution. ABS 043-29 forms an antibody pair for sandwich ELISA with ABS 043-10. It is recommended to use ABS 043-10 as capture antibody and biotinylated ABS 043-29 as a detection antibody.

WB: ABS 043-29 can be used in Western blotting.

IHC-P: ABS 043-29 detects NGAL in paraffin-embedded sections in ischemia-reperfusion injured kidney cells from mouse (dilution 1:1000). Microwave pretreatment using Citrat buffer pH 6, is recommended.

NGAL expression in the ischemia-reperfusion injured mouse kidney;detected with anti-mouse NGAL antibody (ABS 043-29; antibody dilution 1:1000). Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.

NGAL expression 2 days after the introduction of ischemic-reperfusion injured mouse kidney;detected with anti-mouse NGAL antibody (ABS 043-29; antibody dilution 1:1000). Image and data supplied by Prof. Gueler, Medizinische Hochschule Hannover.

1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A

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Product Specification

NGAL Product Overview

Non-hazardous statement (SDS)

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