Mouse monoclonal antibody

Anti-Peptide Histidine Methionine (PHM)

CAT NO: ABS 020-03 | Application: ELISA, IHC

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Product Summary

Cat. No.
ABS 020-03
Specificity
ABS 020-03 binds human PHM and cross-reacts with PACAP (approximately 12%; incomplete, non-parallel inhibition). ABS 020-03 does not cross-react with GLP-1, GLP-2, VIP or glucagon.
Immunogen
Synthetic human peptide histidine-methionine
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG2a/k
Gene ID
7432
Clone Number
3
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. ABS 020-03-04 1 mL: Cat. No. ABS 020-03-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Human peptide histidine-methionine (PHM) is a 27 amino acid peptide belonging to the glucagon-secretin family of peptide hormones. PHM is produced from the same prohormone as VIP, with which it shows considerable sequence homology. The VIP/PHM prohormone is produced by neurons in the central and peripheral nervous systems, often together with acetylcholine. In some situations the effect of the peptides is to reinforce and prolong the effects of neurotransmitter release. Like VIP, PHM is a vasodilator and stimulator of intestinal fluid secretion. High plasma levels of PHM are diagnostic of the corresponding peptide-producing tumor (VIPoma), associated with the watery diarrhea-hypokalemia-achlorhydria syndrome.

Application

ELISA: ABS 020-03 binds free PHM in solution. An ELISA dilution guideline of 1/2000 applies to PHM coated directly at 2 μg/mL. For glutaraldehyde-linked PHM-ovalbumin (molar ratio 4:1) coated at 1 μg/mL the dilution is 1/30,000.

IHC: ABS 020-03 was used in IHC on a human appendix section.

IHC staining of human appendix section. Plasma of the veins stained strongly as well as ganglion cells of neuronal plexuses and neuronal axons. Dilution: 1:200

Cat. No.
ABS 020-03
Specificity
ABS 020-03 binds human PHM and cross-reacts with PACAP (approximately 12%; incomplete, non-parallel inhibition). ABS 020-03 does not cross-react with GLP-1, GLP-2, VIP or glucagon.
Immunogen
Synthetic human peptide histidine-methionine
Host
Mouse
Positive Species Reactivity
Human
Negative Species Reactivity
Not determined
Isotype
IgG2a/k
Gene ID
7432
Clone Number
3
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. ABS 020-03-04 1 mL: Cat. No. ABS 020-03-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Human peptide histidine-methionine (PHM) is a 27 amino acid peptide belonging to the glucagon-secretin family of peptide hormones. PHM is produced from the same prohormone as VIP, with which it shows considerable sequence homology. The VIP/PHM prohormone is produced by neurons in the central and peripheral nervous systems, often together with acetylcholine. In some situations the effect of the peptides is to reinforce and prolong the effects of neurotransmitter release. Like VIP, PHM is a vasodilator and stimulator of intestinal fluid secretion. High plasma levels of PHM are diagnostic of the corresponding peptide-producing tumor (VIPoma), associated with the watery diarrhea-hypokalemia-achlorhydria syndrome.

ELISA: ABS 020-03 binds free PHM in solution. An ELISA dilution guideline of 1/2000 applies to PHM coated directly at 2 μg/mL. For glutaraldehyde-linked PHM-ovalbumin (molar ratio 4:1) coated at 1 μg/mL the dilution is 1/30,000.

IHC: ABS 020-03 was used in IHC on a human appendix section.

IHC staining of human appendix section. Plasma of the veins stained strongly as well as ganglion cells of neuronal plexuses and neuronal axons. Dilution: 1:200

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