Mouse monoclonal antibody

Anti-Perlecan (bovine, human)

CAT NO: CSI 001-76 | Application: ELISA, WB, IHC-F, IHC-P, AP, IP

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Product Summary

Cat. No.
CSI 001-76
Specificity
CSI 001-76 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
Immunogen
Lysed bovine corneal endothelial cells and extracellular matrix
Host
Mouse
Positive Species Reactivity
Bovine, Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
444872, 3339
Clone Number
A76
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. CSI 001-76-04 1 mL: Cat. No. CSI 001-76-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling. (1)

Application

ELISA: CSI 001-76 can be used in ELISA.

WB: CSI 001-76 can be used in Western blotting. (1)

IHC-F: CSI 001-76 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.

IHC-P: CSI 001-76 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.

AP: CSI 001-76 is more sensitive than CSI 001-71 or CSI 001-74 in ELISA. (2)

IP: CSI 001-76 can be used in immunoprecipitation.

Scientific References

1. Whitelock JM, Graham LD, Melrose J, Murdoch AD, Iozzo R, Underwood PA (1999) Human perlecan immunopurified from different endothelial cell sources has different adhesive properties for vascular cells. Matrix Biol 18:163-178.

2. Whitelock JM, Murdoch AD, Iozzo RV, Underwood PA (1996) The degradation of human endothelial cell-derived perlecan, and release of bound bFGF by stromelysin, plasmin and heparanases. J Biol Chem 271:10079-10086.

Cat. No.
CSI 001-76
Specificity
CSI 001-76 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
Immunogen
Lysed bovine corneal endothelial cells and extracellular matrix
Host
Mouse
Positive Species Reactivity
Bovine, Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
444872, 3339
Clone Number
A76
Epitope Specificity
Not determined
Unit Size
400 µL: Cat. No. CSI 001-76-04 1 mL: Cat. No. CSI 001-76-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling. (1)

ELISA: CSI 001-76 can be used in ELISA.

WB: CSI 001-76 can be used in Western blotting. (1)

IHC-F: CSI 001-76 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.

IHC-P: CSI 001-76 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.

AP: CSI 001-76 is more sensitive than CSI 001-71 or CSI 001-74 in ELISA. (2)

IP: CSI 001-76 can be used in immunoprecipitation.

1. Whitelock JM, Graham LD, Melrose J, Murdoch AD, Iozzo R, Underwood PA (1999) Human perlecan immunopurified from different endothelial cell sources has different adhesive properties for vascular cells. Matrix Biol 18:163-178.

2. Whitelock JM, Murdoch AD, Iozzo RV, Underwood PA (1996) The degradation of human endothelial cell-derived perlecan, and release of bound bFGF by stromelysin, plasmin and heparanases. J Biol Chem 271:10079-10086.

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Product Specification

Non-hazardous statement (SDS)

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