Mouse monoclonal antibody

Anti-Perlecan (bovine, human)

CAT NO: CSI 001-74 | Application: ELISA, WB, IHC-F, IHC-P, AP, IP

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Product Summary

Cat. No.

CSI 001-74

Specificity

CSI 001-74 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).

Immunogen

Bovine corneal endothelial cells

Host

Mouse

Positive Species Reactivity

Bovine, Human

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

444872, 3339

Clone Number

A74

Epitope Specificity

Epitope is located in domain V

Unit Size

400 µL: Cat. No. CSI 001-74-04 1 mL: Cat. No. CSI 001-74-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling.

Application

ELISA: CSI 001-74 can be used in ELISA.

WB: In Western blotting a dilution guideline of 1/100 has proved successful. (1)

IHC-F: CSI 001-74 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.

IHC-P: CSI 001-74 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.

AP: CSI 001-74 can be used in affinity chromatography, in conjunction with CSI 001-71 to separate recombinant domain I from full-length perlecan. (1)

IP: CSI 001-74 can be used in immunoprecipitation.

Scientific References

1. Whitelock JM, Murdoch AD, Iozzo RV, Underwood PA (1996) The degradation of human endothelial cell-derived perlecan, and release of bound bFGF by stromelysin, plasmin and heparanases. J Biol Chem 271:10079-10086.