Mouse monoclonal antibody
Anti-Perlecan (bovine, human)
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Product Summary
Application
ELISA: CSI 001-71 can be used in ELISA.
WB: In Western blotting a dilution guideline of 1/100 has proved successful. (1,2)
IHC-F: CSI 001-71 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.
IHC-P: CSI 001-71 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.
AP: CSI 001-71 can be used in affinity chromatography to separate recombinant domain I from tissue culture fluid. (1)
IP: CSI 001-71 can be used in immunoprecipitation.
Scientific References
1. Whitelock JM, Murdoch AD, Iozzo RV, Underwood PA (1996) The degradation of human endothelial cell-derived perlecan, and release of bound bFGF by stromelysin, plasmin and heparanases. J Biol Chem 271:10079-10086.
2. Muthusamy, A, Cooper, CR, Gomes, RR JR. (2010) Soluble perlecan domain i enhances vacular endothelial growth factor-165 actibity and receptor phosphorylation in human bone marrow endothelial cells, BMC Biochem 11:43. doi: 10.1186/1471-2091-11-43.
ELISA: CSI 001-71 can be used in ELISA.
WB: In Western blotting a dilution guideline of 1/100 has proved successful. (1,2)
IHC-F: CSI 001-71 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.
IHC-P: CSI 001-71 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.
AP: CSI 001-71 can be used in affinity chromatography to separate recombinant domain I from tissue culture fluid. (1)
IP: CSI 001-71 can be used in immunoprecipitation.
1. Whitelock JM, Murdoch AD, Iozzo RV, Underwood PA (1996) The degradation of human endothelial cell-derived perlecan, and release of bound bFGF by stromelysin, plasmin and heparanases. J Biol Chem 271:10079-10086.
2. Muthusamy, A, Cooper, CR, Gomes, RR JR. (2010) Soluble perlecan domain i enhances vacular endothelial growth factor-165 actibity and receptor phosphorylation in human bone marrow endothelial cells, BMC Biochem 11:43. doi: 10.1186/1471-2091-11-43.
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