Mouse monoclonal antibody

Anti-Thrombospondin 1 (bovine, human)

CAT NO: CSI 002-65 | Application: ELISA, IHC-F, IHC-P, IP

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Cat. No.	CSI 002-65
Specificity	CSI 002-65 is highly specific for the low-calcium (0.1 mmol/L) conformation of human TSP1. There is no evidence of cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
Immunogen	Extracellular matrix material from cultured bovine corneal endothelial cells
Host	Mouse
Positive Species Reactivity	Bovine, Human
Negative Species Reactivity	Not determined
Isotype	IgG1/k
Gene ID	7057
Clone Number	A65M
Epitope Specificity	Epitope is only present in thrombospondin prepared in low-calcium (0.1 mmol/L) buffers.
Unit Size	400 µL: Cat. No. CSI 002-65-04 1 mL: Cat. No. CSI 002-65-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification	Protein A/G purified (from culture supernatant)
Form	Liquid
Solvent	0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation	Unconjugated BSA free
Storage	4-8ºC without exposure to light. No precautions necessary during handling.
Target	Thrombospondin 1 (TSP1) is a multidomain, multifunctional glycoprotein from platelets and certain vascular cells which has been shown to play an important role in cell-cell and cell-matrix interactions. TSP1 is a calcium-sensitive, disulfide-bonded trimer with a subunit molecular mass of 150 kDa. TSP1 exists in different conformational states depending on the calcium ion concentration used in the purification buffers. (1)