Mouse monoclonal antibody

Anti-Thrombospondin 1 (bovine, human)

CAT NO: CSI 002-65 | Application: ELISA, IHC-F, IHC-P, IP

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Product Summary

Cat. No.

CSI 002-65

Specificity

CSI 002-65 is highly specific for the low-calcium (0.1 mmol/L) conformation of human TSP1. There is no evidence of cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).

Immunogen

Extracellular matrix material from cultured bovine corneal endothelial cells

Host

Mouse

Positive Species Reactivity

Bovine, Human

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Gene ID

7057

Clone Number

A65M

Epitope Specificity

Epitope is only present in thrombospondin prepared in low-calcium (0.1 mmol/L) buffers.

Unit Size

400 µL: Cat. No. CSI 002-65-04 1 mL: Cat. No. CSI 002-65-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Thrombospondin 1 (TSP1) is a multidomain, multifunctional glycoprotein from platelets and certain vascular cells which has been shown to play an important role in cell-cell and cell-matrix interactions. TSP1 is a calcium-sensitive, disulfide-bonded trimer with a subunit molecular mass of 150 kDa. TSP1 exists in different conformational states depending on the calcium ion concentration used in the purification buffers. (1)

Application

ELISA: CSI 002-65 can be used in ELISA. (1)

IHC-F: CSI 002-65 for immunostaining of frozen periodate-lysine-paraformaldehyde-fixed sections of bovine and human tissues. CSI 002-65 can also be used to probe for the low-calcium conformation of thrombospondin. (1)

IHC-P: CSI 002-65 for immunostaining of frozen periodate-lysine-paraformaldehyde-fixed sections of bovine and human tissues. CSI 002-65 can also be used to probe for the low-calcium conformation of thrombospondin. (1)

IP: CSI 002-65 can be used for immunoprecipitation.

Scientific References

1. Matthias LJ, Gotis-Graham I, Underwood PA, McNeil HP, Hogg PJ (1996) Identification of monoclonal antibodies that recognize different disulphide bonded forms of thrombospondin 1. Biochem Biophys Acta 1216:138-144.