Mouse monoclonal antibody

Anti-Thrombospondin 1 (bovine, human)

CAT NO: CSI 002-65 | Application: ELISA, IHC-F, IHC-P, IP

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Product Summary

Cat. No.
CSI 002-65
Specificity
CSI 002-65 is highly specific for the low-calcium (0.1 mmol/L) conformation of human TSP1. There is no evidence of cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
Immunogen
Extracellular matrix material from cultured bovine corneal endothelial cells
Host
Mouse
Positive Species Reactivity
Bovine, Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
7057
Clone Number
A65M
Epitope Specificity
Epitope is only present in thrombospondin prepared in low-calcium (0.1 mmol/L) buffers.
Unit Size
400 µL: Cat. No. CSI 002-65-04 1 mL: Cat. No. CSI 002-65-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Thrombospondin 1 (TSP1) is a multidomain, multifunctional glycoprotein from platelets and certain vascular cells which has been shown to play an important role in cell-cell and cell-matrix interactions. TSP1 is a calcium-sensitive, disulfide-bonded trimer with a subunit molecular mass of 150 kDa. TSP1 exists in different conformational states depending on the calcium ion concentration used in the purification buffers. (1)

Application

ELISA: CSI 002-65 can be used in ELISA. (1)

IHC-F: CSI 002-65 for immunostaining of frozen periodate-lysine-paraformaldehyde-fixed sections of bovine and human tissues. CSI 002-65 can also be used to probe for the low-calcium conformation of thrombospondin. (1)

IHC-P: CSI 002-65 for immunostaining of frozen periodate-lysine-paraformaldehyde-fixed sections of bovine and human tissues. CSI 002-65 can also be used to probe for the low-calcium conformation of thrombospondin. (1)

IP: CSI 002-65 can be used for immunoprecipitation.

Scientific References

1. Matthias LJ, Gotis-Graham I, Underwood PA, McNeil HP, Hogg PJ (1996) Identification of monoclonal antibodies that recognize different disulphide bonded forms of thrombospondin 1. Biochem Biophys Acta 1216:138-144.

Cat. No.
CSI 002-65
Specificity
CSI 002-65 is highly specific for the low-calcium (0.1 mmol/L) conformation of human TSP1. There is no evidence of cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
Immunogen
Extracellular matrix material from cultured bovine corneal endothelial cells
Host
Mouse
Positive Species Reactivity
Bovine, Human
Negative Species Reactivity
Not determined
Isotype
IgG1/k
Gene ID
7057
Clone Number
A65M
Epitope Specificity
Epitope is only present in thrombospondin prepared in low-calcium (0.1 mmol/L) buffers.
Unit Size
400 µL: Cat. No. CSI 002-65-04 1 mL: Cat. No. CSI 002-65-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein A/G purified (from culture supernatant)
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Conjugation
Unconjugated BSA free
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Thrombospondin 1 (TSP1) is a multidomain, multifunctional glycoprotein from platelets and certain vascular cells which has been shown to play an important role in cell-cell and cell-matrix interactions. TSP1 is a calcium-sensitive, disulfide-bonded trimer with a subunit molecular mass of 150 kDa. TSP1 exists in different conformational states depending on the calcium ion concentration used in the purification buffers. (1)

ELISA: CSI 002-65 can be used in ELISA. (1)

IHC-F: CSI 002-65 for immunostaining of frozen periodate-lysine-paraformaldehyde-fixed sections of bovine and human tissues. CSI 002-65 can also be used to probe for the low-calcium conformation of thrombospondin. (1)

IHC-P: CSI 002-65 for immunostaining of frozen periodate-lysine-paraformaldehyde-fixed sections of bovine and human tissues. CSI 002-65 can also be used to probe for the low-calcium conformation of thrombospondin. (1)

IP: CSI 002-65 can be used for immunoprecipitation.

1. Matthias LJ, Gotis-Graham I, Underwood PA, McNeil HP, Hogg PJ (1996) Identification of monoclonal antibodies that recognize different disulphide bonded forms of thrombospondin 1. Biochem Biophys Acta 1216:138-144.

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