Mouse monoclonal antibody

Anti-yENaC (y−subunit epithelial sodium channel)

CAT NO: CAM 005-02 | Application: ELISA, WB, IHC-P, IHC-F, IF

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Product Summary

Cat. No.

CAM 005-02

Specificity

CAM 005-02 is specific for the inhibitory tract of human yENaC subunit.

Immunogen

The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human yENaC subunit.

Host

Mouse

Positive Species Reactivity

Human

Negative Species Reactivity

Not determined

Isotype

IgG1/k

Clone Number

5c2

Epitope Specificity

The epitope is on the inhibitory tract of human yENaC. The epitope differs from that of CAM 003-07.

Unit Size

400 µL: Cat. No. CAM 005-02-04 1 mL: Cat. No. CAM 005-02-1 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

protein A/G purified (from culture supernatant)

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Conjugation

Unconjugated BSA free

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the g-subunit and putative release of a 43-amino acid inhibitory tract from the g-subunit ectodomain.

Application

ELISA: A sandwich ELISA can be made using CAM 003-07 (1-4 μg/ml) as the capture antibody and biotinylated CAM 005-02 (0.05 - 0.2 μg/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131).

WB: CAM 005-02 was used in Western blot. (1)

IHC-P: CAM 005-02 was used in immunohistochemistry. (1)

IHC-F: CAM 005-02 was used in immunohistochemistry. (1)

IF: CAM 005-02 was used in immunofluorescence. (1)

Scientific References

1. Zachar RM, Skjødt K, Marcussen N, Walter S, Toft A, Nielsen MR, Jensen BL, Svenningsen P. The Epithelial Sodium Channel g-Subunit Is Processed Proteolytically in Human Kidney. J Am Soc Nephrol. 2014 Jul 24. pii: ASN.2013111173. (epub ahead of print).